Hansrani Monica, Stansby Gerard
Northern Vascular Unit, Freeman Hospital, Newcastle upon Tyne, UK.
Ann Clin Biochem. 2007 Jul;44(Pt 4):388-90. doi: 10.1258/000456307780945732.
To assess the effectiveness of using 3-deazaadenosine (3DAA) to maintain plasma homocysteine concentrations (tHCy) in whole blood samples.
Blood was obtained from five volunteers and samples were maintained at room temperature, in cold packs or in a fridge (0-4 degrees C) with and without 3DAA. At time points ranging from 6 to 168 h, samples were processed and analysed for tHCy using the Abbott IMx system.
There was a mean increase in tHCy of 29.4% at 6 h increasing to 242.6% after 168 h in whole blood kept at room temperature. There was no significant change in tHCy for 48 h when stored in cold packs, and for 72 h when stored in the fridge. The addition of 3DAA had a significant preservative effect (P<0.001), maintaining tHCy to 48 h in whole blood at room temperature, 120 h in the fridge and 96 h in cool packs. There was no statistical difference in results obtained from samples containing preservative and controls when using the Abbott IMx system.
3DAA is an effective preservative of tHCy in whole blood, particularly in samples maintained at 0-4 degrees C.
评估使用3-去氮腺苷（3DAA）维持全血样本中血浆同型半胱氨酸浓度（tHCy）的有效性。
从五名志愿者身上采集血液，样本分别在室温、冷藏包或冰箱（0-4摄氏度）中保存，添加或不添加3DAA。在6至168小时的时间点，对样本进行处理，并使用雅培IMx系统分析tHCy。
室温下保存的全血中，tHCy在6小时时平均增加29.4%，168小时后增加到242.6%。保存在冷藏包中的样本，tHCy在48小时内无显著变化；保存在冰箱中的样本，tHCy在72小时内无显著变化。添加3DAA具有显著的保存效果（P<0.001），可使室温下全血中的tHCy维持48小时，冰箱中维持120小时，冷藏包中维持96小时。使用雅培IMx系统时，含防腐剂样本和对照样本的检测结果无统计学差异。
3DAA是全血中tHCy的有效防腐剂，尤其在0-4摄氏度保存的样本中。
