Nair Ratish, Chabhadiya Rajesh, Chanda Sumitra
Dept of Biosciences, Saurashtra University, Rajkot, Gujarat, 360 005, India.
J Herb Pharmacother. 2007;7(1):73-86.
This study was done to investigate the antimicrobial potentiality of the marine algae collected from different coastal regions of Gujarat and screened for the same. Twenty-six marine algae belonging to Rhodophyceae, Chlorophyceae and Phaeophyceae were screened for their potential antibacterial activity against five clinically important bacterial strains, namely Bacillus cereus, Micrococcus flavus, Citrobacter freundii, Klebsiella pneumoniae and Pseudomonas testosterone. Acetone and methanol were used for extraction; and the extracted yield was more when the solvent used was methanol. The antibacterial activity was done by both Agar disc diffusion method and Agar ditch method. The five bacterial strains showed varied response towards marine algal extracts. The most susceptible bacteria was B. cereus followed by K. pneumoniae and C. freundii while the most resistant bacteria were M. flavus and P. testosteroni. Among the 26 algae screened, E. intestinalis was the most potent alga and thus, this alga was selected for further studies. E. intestinalis was extracted in petroleum ether, 1,4-dioxan, acetone, methanol and DMF, and their antibacterial activity was studied against the above-stated five bacterial strains using agar disc method. Maximum extractive value of E. intestinalis was in methanol (2.05%) and minimum was in acetone (0.38%). The most susceptible bacteria was K. pneumoniae and maximum antibacterial activity was shown by petroleum ether extract and minimum was shown by 1,4-dioxan extract. The most resistant bacteria were M. flavus and C. freundii. The MIC values of E. intestinalis extracts ranged from 2500-9.765 microg/0.5 ml against B. cereus and K. pneumoniae. From these results it is concluded that the acetone extract of E. intestinalis is the most potent extract and can be used as a lead molecule in drug discovery in inhibiting some of the bacterial strains. E. intestinalis can be used as a promising novel marine antimicrobial agent in the coming years.
本研究旨在调查从古吉拉特邦不同沿海地区采集的海藻的抗菌潜力并进行筛选。对属于红藻门、绿藻门和褐藻门的26种海藻进行了筛选，以检测它们对5种临床重要细菌菌株的潜在抗菌活性，这5种菌株分别是蜡样芽孢杆菌、微黄微球菌、弗氏柠檬酸杆菌、肺炎克雷伯菌和睾丸酮假单胞菌。使用丙酮和甲醇进行提取；当使用甲醇作为溶剂时，提取产率更高。通过琼脂平板扩散法和琼脂沟法进行抗菌活性检测。这5种细菌菌株对海藻提取物表现出不同的反应。最敏感的细菌是蜡样芽孢杆菌，其次是肺炎克雷伯菌和弗氏柠檬酸杆菌，而最具抗性的细菌是微黄微球菌和睾丸酮假单胞菌。在筛选的26种海藻中，肠浒苔是最具效力的海藻，因此，选择该海藻进行进一步研究。用石油醚、1,4 - 二氧六环、丙酮、甲醇和二甲基甲酰胺对肠浒苔进行提取，并使用琼脂平板法研究其对上述5种细菌菌株的抗菌活性。肠浒苔的最大提取值在甲醇中（2.05%），最小在丙酮中（0.38%）。最敏感的细菌是肺炎克雷伯菌，石油醚提取物显示出最大抗菌活性，1,4 - 二氧六环提取物显示的抗菌活性最小。最具抗性的细菌是微黄微球菌和弗氏柠檬酸杆菌。肠浒苔提取物对蜡样芽孢杆菌和肺炎克雷伯菌的最低抑菌浓度值范围为2500 - 9.765微克/0.5毫升。从这些结果可以得出结论，肠浒苔的丙酮提取物是最具效力的提取物，可作为药物发现中抑制某些细菌菌株的先导分子。在未来几年，肠浒苔可作为一种有前景的新型海洋抗菌剂。
