Zhu Lin, Ye Xun, Tang Guangxin, Zhao Nanming, Gong Yandao, Zhao Yuanli, Zhao Jizong, Zhang Xiufang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and MembraneBiotechnology, Tsinghua University, Beijing 100084, China.
Department of Neurosurgery, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.
J Biomed Mater Res A. 2007 Dec 15;83(4):1165-1175. doi: 10.1002/jbm.a.31401.
The modification on the titanium implant surface is an effective method to improve the biocompatibility of titanium. This article describes efforts to improve implant biocompatibility by applying titania and hydroxyapatite to form a three-layer coating on the titanium surface. This three-layer coating is made up of HA as the top layer (formed by hydrothermal treatment), porous TiO2 as the middle layer (formed by micro-arc oxidation) and a dense TiO2 film as the inner layer (formed by preanodic oxidation). The physicochemical characteristics, cell behavior and in vivo studies were assessed. The physicochemical characteristics were investigated using scanning electron micoscopy observation, fibronectin and laminin adsorption, corrosion test and X-ray diffraction analysis. Cell behavior included morphology observation with scanning electron microscopy (SEM), number count with methylthiazol tetrazolium (MTT) assay and Alkaline phosphatase (ALP, a representative enzyme of osteoblastic differentiation) activity of osteoblast-like MC3T3-E1 cells. In study in vivo the specimens were embedded in skull wound for repair. By the analysis of experiments, the titanium coated with this three-layer coating has been proved to have excellent corrosion resistance and good biocompatibility, which can promote cell proliferation and bone formation. So this modified titanium is an improved alternative to untreated titanium for bone repair applications.
对钛植入体表面进行改性是提高钛生物相容性的有效方法。本文介绍了通过应用二氧化钛和羟基磷灰石在钛表面形成三层涂层来提高植入体生物相容性的研究。这种三层涂层由顶层的HA（通过水热处理形成）、中间层的多孔TiO₂（通过微弧氧化形成）和内层的致密TiO₂膜（通过预阳极氧化形成）组成。对其物理化学特性、细胞行为和体内研究进行了评估。通过扫描电子显微镜观察、纤连蛋白和层粘连蛋白吸附、腐蚀试验以及X射线衍射分析来研究物理化学特性。细胞行为包括用扫描电子显微镜（SEM）观察形态、用甲基噻唑四氮唑（MTT）法计数细胞数量以及成骨样MC3T3-E1细胞的碱性磷酸酶（ALP，成骨细胞分化的代表性酶）活性。在体内研究中，将标本植入颅骨伤口进行修复。通过实验分析，已证明涂覆有这种三层涂层的钛具有优异的耐腐蚀性和良好的生物相容性，能够促进细胞增殖和骨形成。因此，这种改性钛是用于骨修复应用的未处理钛的一种改进替代品。
