使用DNA微阵列检测有和没有细胞学异常的女性中23种人乳头瘤病毒类型的频率

Frequency of 23 human papillomavirus types using DNA microarray in women with and without cytological anomalies.

作者信息

Tempfer Clemens, Grimm Christoph, Harwanegg Christian, Huber Martin, Mueller Manfred W, Buerkle Bernd, Reinthaller Alexander, Hefler Lukas A

机构信息

Department of Obstetrics and Gynecology, University of Vienna School of Medicine, Vienna, Austria.

出版信息

Anticancer Res. 2007 May-Jun;27(3B):1721-6.

PMID:17595804

Abstract

OBJECTIVE

To evaluate the frequency of single and multiple human papillomavirus (HPV) infections in women with and without cervical dysplasia.

MATERIALS AND METHODS

An oligonucleotide microarray system was used to detect 19 types of high-risk HPV (HPV-16/-18/-311-33/-35/-39/-45/-51/ -52/-53/-56/-58/-59/-66/-68/-73/-821-16 variant E-E6-G350/-16 variant E-E6-T350) and 4 types of low-risk HPV (HPV-6/-11/ -42/-44) in 122 consecutive women visiting our colposcopy outpatient clinic classified into controls (normal epithelium, nonspecific cervicitis, metaplasia; n = 56) and cervical intraepithelial neoplasia (CIN) (n = 66).

RESULTS

In 78/122 (64%) cervical samples, HPV DNA was detected. Compared to controls, HPV infection was significantly more prevalent among women with CIN (8/56 [14%] versus 49/66 [74%]; p = 0.001). HPV-18 and HPV-16 were the most common HPV types in all specimens (25% [31/122] and 25% [31/122], respectively). Of note, HPV-16 was significantly more frequent in women with CIN compared to controls (35% [23/66] vs. 14% [8/56], respectively; p = 0.02). Double HPV infections were detected in 16/122 (13%) and multiple infections in 43/122 (35%) women. Multiple HPV infections were found significantly more often among women with CIN compared to controls (30/66 [45%] vs. 13/56 [23%], respectively; p = 0.002). Using a univariate and multivariate logistic regression model to estimate the relative risk of CIN vs. HPV type, HPV-16-positive cases were found to have the highest risk of CIN (odds ratio [OR] 3.2; 95% confidence interval [CI] 1.3-7.9; p = 0.002).

CONCLUSION

Multiple HPV infections are common in women with and without CIN, but significantly more prevalent among women with CIN compared to controls.

摘要

目的

评估有和没有宫颈发育异常的女性中单一和多重人乳头瘤病毒（HPV）感染的频率。

材料与方法

使用寡核苷酸微阵列系统检测122名连续到我们阴道镜门诊就诊的女性中的19种高危型HPV（HPV-16/-18/-31/-33/-35/-39/-45/-51/-52/-53/-56/-58/-59/-66/-68/-73/-82/-16变异体E-E6-G350/-16变异体E-E6-T350）和4种低危型HPV（HPV-6/-11/-42/-44）。这些女性被分为对照组（正常上皮、非特异性宫颈炎、化生；n = 56）和宫颈上皮内瘤变（CIN）组（n = 66）。

结果

在122份宫颈样本中的78份（64%）检测到HPV DNA。与对照组相比，CIN女性中HPV感染明显更普遍（8/56 [14%] 对比49/66 [74%]；p = 0.001）。HPV-18和HPV-16是所有样本中最常见的HPV类型（分别为25% [31/122] 和25% [31/122]）。值得注意的是，与对照组相比，CIN女性中HPV-16的频率明显更高（分别为35% [23/66] 对比14% [8/56]；p = 0.02）。在122名女性中的16名（13%）检测到双重HPV感染，43名（35%）检测到多重感染。与对照组相比，CIN女性中发现多重HPV感染的频率明显更高（分别为30/66 [45%] 对比13/56 [23%]；p = 0.002）。使用单变量和多变量逻辑回归模型估计CIN与HPV类型的相对风险，发现HPV-16阳性病例发生CIN的风险最高（优势比[OR] 3.2；95%置信区间[CI] 1.3 - 7.9；p = 0.002）。