Domeij S, Carlsöö B, Dahlqvist A, Forsgren S
Department of Histology, University of Umea, Sweden.
Acta Otolaryngol. 1991;111(5):981-9. doi: 10.3109/00016489109138440.
The distribution of mast cells in the rat larynx was studied at a light microscopic level in relation to nerve fibers showing substance P (SP)- and calcitonin gene-related peptide (CGRP)-like immunoreactivity (-LI) and ultrastructurally in relation to unmyelinated nerve fibers. Connective tissue mast cells (CTMC), demonstrated by staining for avidin-peroxidase and immunohistochemical staining for 5-hydroxytryptamine (5-HT), were localized in the lamina propria. Alcian blue and toluidine blue revealed mast cells in the epithelium and the lamina propria. The mast cells showed similar regional differences in their distribution as fibers showing SP- and CGRP-LI. A large or moderate number of cells was found on the laryngeal side of the epiglottis, in the ventral recess and in the aryepiglottic folds. No mast cells were found in the vocal cords. In addition, a large number of cells showing 5-HT-LI were observed in the subglottic epithelium but not in the supraglottic and the glottic epithelia. Ultrastructurally and by use of SP- and CGRP-immunohistochemistry, nerve fibers were sometimes observed close to the mast cells. These findings may be of importance in the understanding of laryngeal pathophysiological events.
在光学显微镜水平上，研究了大鼠喉中肥大细胞的分布与显示P物质（SP）和降钙素基因相关肽（CGRP）样免疫反应性（-LI）的神经纤维的关系，并在超微结构水平上研究了与无髓神经纤维的关系。通过抗生物素蛋白-过氧化物酶染色和5-羟色胺（5-HT）免疫组织化学染色显示的结缔组织肥大细胞（CTMC）定位于固有层。阿尔辛蓝和甲苯胺蓝显示上皮和固有层中有肥大细胞。肥大细胞在其分布上显示出与显示SP和CGRP-LI的纤维相似的区域差异。在会厌的喉侧、腹侧隐窝和声襞中发现大量或中等数量的细胞。声带中未发现肥大细胞。此外，在声门下上皮中观察到大量显示5-HT-LI的细胞，但在声门上和声门上皮中未观察到。在超微结构水平上并通过使用SP和CGRP免疫组织化学，有时可观察到靠近肥大细胞的神经纤维。这些发现可能对理解喉部病理生理事件具有重要意义。
