Domeij S, Dahlqvist A, Forsgren S
Department of Histology and Cell Biology, University of Umeå, Sweden.
Anat Embryol (Berl). 1991;183(1):49-56. doi: 10.1007/BF00185834.
The distribution of substance P (SP) - and calcitonin gene-related peptide (CGRP) - containing nerve fibers in the rat larynx was studied using immunohistochemistry. Double-labeling studies revealed a high degree of co-existence of SP- and CGRP-like immunoreactivity (LI) in the nerve fibers in the larynx. There was a considerable regional difference in the number of immunoreactive nerve fibers in the epithelium and lamina propria. Richly supplied sites were the laryngeal side of the epiglottis and the ventral recess, whilst there was no evidence of nerve fibers in the squamous epithelium of the vocal cords. However, where the squamous epithelium of the vocal cords changed into a cuboidal epithelium, a moderate number of nerve fibers was present, and a large number of fibers was seen where the squamous epithelium of the cords was in close contact with cartilage. Nerve fibers showing SP- and CGRP-LI were also observed close to the acini and ducts of the glands, in the blood vessel walls, close to the perichondrium of all the cartilages, and outside the cricothyroid and cricoarytenoid joints. CGRP-LI was detected in epithelial cells facing the lumen of the airway and in cells in the acini and ducts of glands in the subglottic region and trachea. Unilateral sympathectomy did not affect the pattern of SP- and CGRP-innervation in the larynx, whereas after vagotomy, the SP- and CGRP-innervation almost disappeared ipsilaterally in the upper parts of the epiglottis and aryepiglottic folds.
采用免疫组织化学方法研究了大鼠喉中含P物质（SP）和降钙素基因相关肽（CGRP）的神经纤维分布。双重标记研究显示，喉神经纤维中SP样和CGRP样免疫反应性（LI）高度共存。上皮和固有层中免疫反应性神经纤维的数量存在显著的区域差异。供应丰富的部位是会厌喉侧和腹侧隐窝，而声带鳞状上皮中未发现神经纤维。然而，在声带鳞状上皮转变为立方上皮的部位，有适量的神经纤维，在声带鳞状上皮与软骨紧密接触的部位可见大量纤维。在腺泡和导管附近、血管壁、所有软骨的软骨膜附近以及环甲关节和环杓关节外也观察到显示SP和CGRP-LI的神经纤维。在气道腔面的上皮细胞以及声门下区和气管腺泡和导管中的细胞中检测到CGRP-LI。单侧交感神经切除术不影响喉中SP和CGRP的神经支配模式，而迷走神经切断术后，会厌上部和声襞的SP和CGRP神经支配在同侧几乎消失。
