Sun Baocun, Zhang Shiwu, Zhang Danfang, Liu Yanqing, Li Yan, Rong Zhe, Zhu Yue, Jia Xinghong
Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, PR China.
FEBS Lett. 2007 Jul 10;581(17):3277-82. doi: 10.1016/j.febslet.2007.06.021. Epub 2007 Jun 21.
Two-dimensional electrophoresis and Matrix-assisted laser desorption ionization-time of flight-time of mass spectrometry were used to detect the differentially expressed proteins in serum of tientsin albinao 2 mice with spontaneous breast cancer, normal tientsin albinao 2 mice and tientsin albinao 1 mice. Only nuclear clusterin (n-CLU) was expressed in tientsin albinao 1. Immunohistochemistry and western blot validated that n-CLU was present in normal tientsin albinao 2 and tientsin albinao 1 mammary epithelium, and secretory clusterin expressed in the cytoplasm of normal tientsin albinao 2 mammary epithelium and spontaneous breast cancer. n-CLU may play an important role in tientsin albinao 2 spontaneous breast cancer initiation and development.
采用二维电泳和基质辅助激光解吸电离飞行时间质谱技术，检测患有自发性乳腺癌的天津白2小鼠、正常天津白2小鼠和天津白1小鼠血清中差异表达的蛋白质。天津白1小鼠中仅表达核簇蛋白（n-CLU）。免疫组织化学和蛋白质印迹法证实，n-CLU存在于正常天津白2小鼠和天津白1小鼠的乳腺上皮中，而分泌型簇蛋白在正常天津白2小鼠乳腺上皮和自发性乳腺癌的细胞质中表达。n-CLU可能在天津白2小鼠自发性乳腺癌的发生和发展中起重要作用。
