Zanardo Vincenzo, Golin Rosanna, Amato Maurizio, Trevisanuto Daniele, Favaro Flaviano, Faggian Diego, Plebani Mario
Department of Pediatrics, Padua University, 35128 Padua, Italy.
Pediatr Res. 2007 Aug;62(2):191-4. doi: 10.1203/PDR.0b013e31809871c9.
Breast-fed infants have higher bilirubin levels than formula-fed infants, possibly because of variations in the composition of the breast milk. The aim of this study was to investigate whether there is a relationship between cytokine levels in the colostrum of nursing mothers and neonatal jaundice (NJ). Breast milk samples were collected from breast-feeding mothers of healthy full-term neonates, 32 with NJ and 29 without jaundice. The concentrations of IL-1beta, IL-6, IL-8, IL-10, and tumor necrosis factor (TNF)-alpha were measured by chemiluminescence enzyme immunometric assays. Mothers of infants with NJ had a higher concentration of IL-1beta in colostrum, compared with those feeding neonates without NJ, and similar trends were seen for IL-6, IL-8, IL-10, and for TNF-alpha. The concentrations of IL-1beta significantly correlated with IL-6, IL-8, IL-10, and TNF-alpha concentrations, but not with serum bilirubin levels of infants with NJ. In conclusion, the concentrations of IL-1beta were increased in colostrum from breast-feeding mothers whose infants had NJ. The correlation between the concentrations of cytokines involved in the function of hepatic uptake and excretory systems and in the enterohepatic circulation of bilirubin provides additional data to the delineation of the cascade of pathophysiological events that can lead to NJ.
母乳喂养的婴儿比配方奶喂养的婴儿胆红素水平更高，这可能是由于母乳成分的差异所致。本研究的目的是调查哺乳期母亲初乳中的细胞因子水平与新生儿黄疸（NJ）之间是否存在关联。从健康足月儿的母乳喂养母亲中采集母乳样本，其中32例婴儿患有NJ，29例婴儿无黄疸。采用化学发光酶免疫分析法测定白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）和肿瘤坏死因子（TNF）-α的浓度。与喂养无NJ新生儿的母亲相比，患有NJ婴儿的母亲初乳中IL-1β浓度更高，IL-6、IL-8、IL-10和TNF-α也呈现类似趋势。IL-1β的浓度与IL-6、IL-8、IL-10和TNF-α的浓度显著相关，但与患有NJ婴儿的血清胆红素水平无关。总之，婴儿患有NJ的母乳喂养母亲初乳中IL-1β浓度升高。参与肝脏摄取和排泄系统功能以及胆红素肠肝循环的细胞因子浓度之间的相关性，为描绘可导致NJ的病理生理事件级联提供了更多数据。
