Apaydin Kader, Ermis Bahri, Arasli Mehmet, Tekin Ishak, Ankarali Handan
Department of Pediatrics Immunology, School of Medicine, Karaelmas University, Zonguldak, Turkey.
Pediatr Int. 2012 Dec;54(6):801-5. doi: 10.1111/j.1442-200X.2012.03680.x. Epub 2012 Sep 11.
Maternal milk plays an important role in the development of late-onset breast milk jaundice (BMJ), possibly due to the unique characteristics of breast milk. The aim of this study was to investigate whether there is a relation between cytokine concentrations in the milk of nursing mothers and BMJ.
Breast milk samples were collected from breast-feeding mothers of healthy full-term neonates, 40 with BMJ and 40 without jaundice. Milk samples were taken between the second and the fourth postpartum week. The concentrations of interleukin (IL)-1 β, IL-6, IL-8, IL-10, and tumor necrosis factor-α were measured by flow cytometric bead array.
There were significant differences between the study groups in terms of IL-1 β concentrations (P= 0.013). Not statistically significant but similar trends were also seen for IL-10 (P= 0.067) and tumor necrosis factor-α (P= 0.053) concentrations. However, no significant differences were noted in IL-6 (P= 0.174) and IL-8 (P= 0.285) concentrations.
IL-1 β concentration seems to be increased in milk of mothers whose infants had BMJ. Although the effect of these cytokines on BMJ is unknown, it may cause prolonged jaundice via hepatic uptake, hepatic excretion, conjugation and intestinal absorption.
母乳在晚发性母乳性黄疸（BMJ）的发生发展中起重要作用，可能归因于母乳的独特特性。本研究的目的是调查哺乳母亲乳汁中细胞因子浓度与BMJ之间是否存在关联。
从健康足月新生儿的母乳喂养母亲中收集母乳样本，其中40例婴儿患有BMJ，40例无黄疸。在产后第二至第四周采集乳汁样本。采用流式细胞仪微球阵列法检测白细胞介素（IL）-1β、IL-6、IL-8、IL-10和肿瘤坏死因子-α的浓度。
研究组之间IL-1β浓度存在显著差异（P = 0.013）。IL-10（P = 0.067）和肿瘤坏死因子-α（P = 0.053）浓度虽无统计学意义，但也呈现相似趋势。然而，IL-6（P = 0.174）和IL-8（P = 0.285）浓度无显著差异。
婴儿患有BMJ的母亲乳汁中IL-1β浓度似乎升高。尽管这些细胞因子对BMJ的影响尚不清楚，但它们可能通过肝脏摄取、肝脏排泄、结合和肠道吸收导致黄疸持续时间延长。
