Katalinić Visnja, Salamunić Ilza, Pazanin Senijo, Mulić Rosanda, Milisić Marija, Ropac Darko
Department of Food Technology and Biotechnology, Faculty of Chemical Technology, University of Split, Split, Croatia.
Coll Antropol. 2007 Mar;31(1):165-71.
The aim of this study was to determine the antioxidant potential of the serum and the level of lipid oxidation products in the sera of apparently healthy adult males. The "antioxidant power" of the serum, defined as the ability to reduce ferric ions by antioxidants from the serum (FRAP), was taken as the indicator of total antioxidation potential. The formation of lipid oxidation products was evaluated as thiobarbituric reactive species serum test (TBARS). The ferrous oxidation in xylenol orange version 2 (FOX2) assay coupled with triphenylphosphine was used for measurement of total sera hydroperoxides (ROOHs). The following biochemical variables were determined in the sera: aspartat aminotranspherase (AST), alanine aminotranspherase (ALT), gamma-glutamyl transpherase (GGT), bilirubin, glucose, creatinine, cholesterol, triglycerides and hemoglobin. Blood sera from apparently healthy subjects (166 adult males) were analyzed. Median age of study participants was 36 years (range 25-50 years). The X +/- SD sera FRAP level of the 166 apparently healthy adult males was 1047 +/- 131 micromol/L (779-1410 range). The X +/- SD level of sera TBARS was 1.2 +/- 0.3 micromol/L of the sera (0.5-2.2 range). Compared with the level of TBARS in the sera, the level of FOX2-ROOH was significantly higher The X +/- SD level of lipid hydroperoxides in the fresh sera, determined as FOX2, was 3.9 +/- 1.5 micromol/L of the sera (1.9-6.9 range). Observation of correlation between FRAP and determined biochemical variables in the sera have confirmed a statistically significant linear correlation between sera FRAP and bilirubin, hemoglobine, glucose, ALT and triglycerides (p < 0.05). The results of sera FRAP, TBARS and FOX2 levels can help in estimating the antioxidant status of humans. Significant correlation between the antioxidant power of blood serum and particular biochemical parameters indicates the complexity of defence mechanisms and various molecules involved in increasing the reduction power of the serum.
本研究的目的是确定貌似健康的成年男性血清的抗氧化潜力以及血清中脂质氧化产物的水平。血清的“抗氧化能力”定义为血清中的抗氧化剂还原铁离子的能力（FRAP），被用作总抗氧化潜力的指标。脂质氧化产物的形成通过硫代巴比妥酸反应性物质血清试验（TBARS）进行评估。二甲苯酚橙版本2（FOX2）法结合三苯基膦用于测定血清总氢过氧化物（ROOHs）。在血清中测定了以下生化变量：天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（GGT）、胆红素、葡萄糖、肌酐、胆固醇、甘油三酯和血红蛋白。对貌似健康的受试者（166名成年男性）的血清进行了分析。研究参与者的中位年龄为36岁（范围25 - 50岁）。166名貌似健康的成年男性的血清FRAP水平X±SD为1047±131微摩尔/升（范围779 - 1410）。血清TBARS水平X±SD为1.2±0.3微摩尔/升（范围0.5 - 2.2）。与血清中TBARS水平相比，FOX2 - ROOH水平显著更高。新鲜血清中脂质氢过氧化物的水平（以FOX2测定）X±SD为3.9±1.5微摩尔/升（范围1.9 - 6.9）。对血清FRAP与测定的生化变量之间相关性的观察证实，血清FRAP与胆红素、血红蛋白、葡萄糖、ALT和甘油三酯之间存在统计学上显著的线性相关性（p < 0.05）。血清FRAP、TBARS和FOX2水平的结果有助于评估人类的抗氧化状态。血清抗氧化能力与特定生化参数之间的显著相关性表明了防御机制以及参与提高血清还原能力的各种分子的复杂性。
