Kim Namshin, Lee Christopher
Center for Computational Biology, Molecular Biology Institute, University of California, Los Angeles, 90095-1570, USA.
Bioinformatics. 2007 Sep 1;23(17):2331-3. doi: 10.1093/bioinformatics/btm343. Epub 2007 Jun 28.
We have developed a quick web-based application for designing conserved genomic PCR and RT-PCR primers from multigenome alignments targeting specific exons or introns. We used Pygr (The Python Graph Database Framework for Bioinformatics) to query intervals from multigenome alignments, which gives us less than a millisecond access to any intervals of any genome within multigenome alignments. PRIMER3 was used to extract optimal primers from a gene of interest. QPRIMER creates an electronic genomic PCR image from a set of conserved primers as well as summary pages for primer alignments and products. QPRIMER supports human, mouse, rat, chicken, dog, zebrafish and fruit fly.
我们开发了一个基于网络的快速应用程序，用于从多基因组比对中设计针对特定外显子或内含子的保守基因组PCR和RT-PCR引物。我们使用Pygr（用于生物信息学的Python图形数据库框架）从多基因组比对中查询区间，这使我们能够在不到一毫秒的时间内访问多基因组比对中任何基因组的任何区间。PRIMER3用于从感兴趣的基因中提取最佳引物。QPRIMER根据一组保守引物创建电子基因组PCR图像以及引物比对和产物的汇总页面。QPRIMER支持人类、小鼠、大鼠、鸡、狗、斑马鱼和果蝇。
