Capoen Ward, Den Herder Jeroen, Rombauts Stephane, De Gussem Jeroen, De Keyser Annick, Holsters Marcelle, Goormachtig Sofie
Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent University, B-9052 Ghent, Belgium.
Plant Physiol. 2007 Aug;144(4):1878-89. doi: 10.1104/pp.107.102178. Epub 2007 Jun 28.
The tropical legume Sesbania rostrata provides its microsymbiont Azorhizobium caulinodans with versatile invasion strategies to allow nodule formation in temporarily flooded habitats. In aerated soils, the bacteria enter via the root hair curling mechanism. Submergence prevents this epidermal invasion by accumulation of inhibiting concentrations of ethylene and, under these conditions, the bacterial colonization occurs via intercellular cortical infection at lateral root bases. The transcriptome of both invasion ways was compared by cDNA-amplified fragment length polymorphism analysis. Clusters of gene tags were identified that were specific for either epidermal or cortical invasion or were shared by both. The data provide insight into mechanisms that control infection and illustrate that entry via the epidermis adds a layer of complexity to rhizobial invasion.
热带豆科植物喙荚田菁为其微共生体茎瘤固氮根瘤菌提供了多种侵入策略，以便在临时水淹的生境中形成根瘤。在通气良好的土壤中，细菌通过根毛卷曲机制进入。水淹会因乙烯浓度积累抑制表皮侵入，在此条件下，细菌通过侧根基部的细胞间皮层感染进行定殖。通过cDNA扩增片段长度多态性分析比较了两种侵入方式的转录组。鉴定出了特定于表皮或皮层侵入的基因标签簇，或两者共有的基因标签簇。这些数据为控制感染的机制提供了见解，并表明通过表皮进入增加了根瘤菌侵入的复杂性。
