Kopf Eliezer, Zharhary Dorit
Sigma-Aldrich Israel Ltd., 3 Plaut Street, Park Rabin, Rehovot 76100, Israel.
Int J Biochem Cell Biol. 2007;39(7-8):1305-17. doi: 10.1016/j.biocel.2007.04.029. Epub 2007 May 13.
Cancer is a result of complex changes that occur in normal cells as they transform to become malignant and further when they become metastatic. These changes are not a consequence of a single protein but rather involve multiple proteins that function in pathways and networks. Thus, profiling cancer-associated changes requires simultaneous measurement of many proteins in a single sample. Identifying these changes may lead to the discovery of cancer-associated biomarkers that may assist in diagnosis, prognosis, patient monitoring and possibly for therapeutic purposes. Antibody arrays are a relatively new technology that enables one to perform multiplex high-throughput protein expression profiling. This review describes current technologies in antibody array and assay design, and presents a survey of the current literature on the use of these arrays in cancer research.
癌症是正常细胞发生复杂变化的结果，这些细胞先转变为恶性细胞，进而发生转移。这些变化并非单一蛋白质所致，而是涉及在信号通路和网络中发挥作用的多种蛋白质。因此，分析癌症相关变化需要在单个样本中同时检测多种蛋白质。识别这些变化可能会发现有助于诊断、预后评估、患者监测以及可能用于治疗目的的癌症相关生物标志物。抗体阵列是一项相对较新的技术，可实现多重高通量蛋白质表达谱分析。本文综述了抗体阵列和检测设计的当前技术，并对这些阵列在癌症研究中的应用的当前文献进行了综述。
