Evans R C, Holmes C J
Renal Division, Baxter Healthcare Corp., Round Lake, Ill.
Blood Purif. 1991;9(2):92-101. doi: 10.1159/000170002.
The in vitro transfer of cytokine-inducing substances (CIS) across cellulose triacetate and polyacrylonitrile hollow-fiber high-flux hemodialyzers was studied using culture filtrates of gram-negative bacteria isolated from hemodialysis center environments. With Enterobacter cloacae, no transfer of CIS was seen despite the potent cytokine inducibility and endotoxin content of the challenge solution. In contrast, interleukins 1 and 6 and tumor necrosis factor inducing substances did penetrate both dialyzer types challenged with Pseudomonas aeruginosa culture filtrates containing a high endotoxin content. Transfer was not seen, however, upon dilution of the challenge solution to lower, yet clinically very high levels of endotoxin. These results show that, in vitro, the transfer of CIS across high-flux membranes is critically dependent upon the quality and the quantity of the challenge material employed.
使用从血液透析中心环境中分离出的革兰氏阴性菌培养滤液，研究了细胞因子诱导物质（CIS）在三醋酸纤维素和聚丙烯腈中空纤维高通量血液透析器中的体外转运情况。对于阴沟肠杆菌，尽管激发溶液具有强大的细胞因子诱导能力和内毒素含量，但未观察到CIS的转运。相比之下，白细胞介素1和6以及肿瘤坏死因子诱导物质确实穿透了用含有高内毒素含量的铜绿假单胞菌培养滤液挑战的两种透析器类型。然而，当将激发溶液稀释至较低但临床上仍非常高的内毒素水平时，未观察到转运。这些结果表明，在体外，CIS跨高通量膜的转运严重依赖于所用激发材料的质量和数量。
