Ambrosone Christine B, Abrams Sara M, Gorlewska-Roberts Katarzyna, Kadlubar Fred F
Division of Epidemiology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Arch Biochem Biophys. 2007 Aug 15;464(2):169-75. doi: 10.1016/j.abb.2007.05.018. Epub 2007 Jun 8.
Diet and environmental exposures to aromatic and heterocyclic amines, and polycyclic aromatic hydrocarbons, are thought to be etiologic factors for breast cancer risk. In this study, we chose to quantify the major DNA adduct derived from one member of each of these classes of carcinogens in epithelial cell DNA isolated from human breast milk. Appreciable adducts were detected for each class, namely 2-amino-6-phenylimidazo[4,5-b]pyridine (PhIP), 4-aminobiphenyl (ABP) and benzo[a]pyrene. The effect of several metabolic genotypes on adduct levels were also investigated and higher PhIP and ABP adducts were associated with the rapid NAT2 and/or rapid NAT1 genotypes. The presence of ABP adducts was also significantly associated with the use of hair coloring products (OR=11.2, 95% CI=1.1-109.2) but not tobacco usage. These data indicate that women are exposed to several classes of dietary and environmental carcinogens and that metabolic genotype can be a susceptibility factor.
饮食以及环境中接触芳香胺、杂环胺和多环芳烃被认为是乳腺癌风险的病因。在本研究中，我们选择对从人母乳中分离的上皮细胞DNA中，这些致癌物类别中每一类的一种成分所衍生的主要DNA加合物进行定量。每一类均检测到了可观的加合物，即2-氨基-6-苯基咪唑并[4,5-b]吡啶（PhIP）、4-氨基联苯（ABP）和苯并[a]芘。还研究了几种代谢基因型对加合物水平的影响，较高的PhIP和ABP加合物与快速NAT2和/或快速NAT1基因型相关。ABP加合物的存在也与使用染发产品显著相关（比值比=11.2，95%置信区间=1.1-109.2），但与吸烟无关。这些数据表明，女性接触多种饮食和环境致癌物，并且代谢基因型可能是一个易感因素。
