肺炎球菌隐蔽期复合物主要蛋白质成分的鉴定
Identification of the major protein component of the pneumococcal eclipse complex.
作者信息
Morrison Donald A, Mortier-Barrière Isabelle, Attaiech Laetitia, Claverys Jean-Pierre
机构信息
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Ave., Chicago, IL 60607, USA.
出版信息
J Bacteriol. 2007 Sep;189(17):6497-500. doi: 10.1128/JB.00687-07. Epub 2007 Jun 29.
Abstract
During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.
摘要
在肺炎链球菌的基因转化过程中，来自天然供体DNA的单链进入感受态细胞，在那里它们与一种分子量为15至20 kDa的未知蛋白质结合形成蚀复合物。通过蛋白质免疫印迹法，我们确定在该复合物中与供体DNA共分离的主要蛋白质为SsbB。
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