肺炎球菌中的转化：涉及处于隐蔽期的供体脱氧核糖核酸单链的复合物的存在及其性质
Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.
作者信息
Morrison D A
出版信息
J Bacteriol. 1977 Nov;132(2):576-83. doi: 10.1128/jb.132.2.576-583.1977.
Abstract
Donor deoxyribonucleic acid (DNA) single strands exist in a complex during the eclipse phase in pneumococcal transformation. This eclipse complex exhibited specific physical properties distinct from those of both pure DNA single strands and native DNA. These included a lower affinity for diethylaminoethyl-cellulose and hydroxylapatite than that of single-strand DNA, faster sedimentation than the DNA chains that it contains, and a buoyant density in Cs2SO4 lower than that of native DNA. The complex was dissociated by treatments with sodium dodecyl sulfate, NaOH, guanidine-hydrochloride, chloroform, and proteinase K but was insensitive to ribonuclease.
摘要
在肺炎球菌转化的隐蔽期，供体脱氧核糖核酸（DNA）单链以复合物形式存在。这种隐蔽复合物表现出与纯DNA单链和天然DNA不同的特定物理性质。这些性质包括对二乙氨基乙基纤维素和羟基磷灰石的亲和力低于单链DNA，沉降速度比其所含的DNA链快，以及在硫酸铯中的浮力密度低于天然DNA。该复合物可通过用十二烷基硫酸钠、氢氧化钠、盐酸胍、氯仿和蛋白酶K处理而解离，但对核糖核酸酶不敏感。
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