Mendes-Ferreira A, del Olmo M, García-Martínez J, Jiménez-Martí E, Leão C, Mendes-Faia A, Pérez-Ortín J E
Centro de Genética e Biotecnologia-IBB, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal.
Appl Environ Microbiol. 2007 Aug;73(16):5363-9. doi: 10.1128/AEM.01029-07. Epub 2007 Jun 29.
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.
对葡萄酒酵母菌株酿酒酵母PYCC4072进行全基因组分析,结果表明,与氮充足条件相比,在低氮或无氮条件下有36个基因高度表达。用该菌株对其中四个转录本进行逆转录PCR分析,并在另一个葡萄酒酵母菌株上进行验证,强调了这些特征基因在预测氮缺乏以及因此诊断葡萄酒发酵停滞/缓慢方面的有用性。