秀丽隐杆线虫中细胞特异性转基因表达的时间控制。
Temporal control of cell-specific transgene expression in Caenorhabditis elegans.
作者信息
Bacaj Taulant, Shaham Shai
机构信息
Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
出版信息
Genetics. 2007 Aug;176(4):2651-5. doi: 10.1534/genetics.107.074369. Epub 2007 Jul 1.
Abstract
Cell-specific promoters allow only spatial control of transgene expression in Caenorhabditis elegans. We describe a method, using cell-specific rescue of heat-shock factor-1 (hsf-1) mutants, that allows spatial and temporal regulation of transgene expression. We demonstrate the utility of this method for timed reporter gene expression and for temporal studies of gene function.
摘要
细胞特异性启动子仅能对线虫中的转基因表达进行空间控制。我们描述了一种利用热休克因子-1（hsf-1）突变体的细胞特异性拯救方法，该方法能对转基因表达进行空间和时间调控。我们展示了此方法在定时报告基因表达及基因功能的时间研究中的效用。
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