Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York 10016.
King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), KAUST Environmental Epigenetics Program (KEEP), Thuwal 23955-6900, Saudi Arabia
Genetics. 2019 Aug;212(4):959-990. doi: 10.1534/genetics.119.301506.
The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made very useful for understanding, , developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in , with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression.
任何遗传模式生物的力量都部分源于其基因表达的可操作性。因其世代时间短、发育谱系不变， 非常有助于理解发育程序、基础细胞生物学、神经生物学和衰老。在过去的十年中，转基因工具包得到了极大的扩展，增加了各种控制表达和以空前分辨率修饰基因的方法。在这里，我们提供了一个在 中使用的转基因方法的全面概述，重点介绍了转座子介导的转基因、CRISPR/Cas9 基因编辑、条件基因和蛋白质失活以及用于表达的时空控制的二部分系统等方面的最新进展。
