Yin Jiangmei, Jin Huali, Yang Fu, Ding Zheng, Huang Chang, Zhu Qinghong, Wang Bin
State Key Laboratory for Agro-Biotechnology, Key Laboratory of Agro-Microbial Resource, and Application of MOA, College of Biology, China Agricultural University, Beijing, China.
Viral Immunol. 2007 Summer;20(2):288-99. doi: 10.1089/vim.2006.0108.
Both humoral and cell-mediated immune responses are important to protect animals from initial acute viral infection and establishment of chronic infection. Adjuvants for DNA vaccines can influence the balance between humoral and cell-mediated immunities. In this study, a DNA vaccine encoding the hemagglutinin-neuraminidase and fusion genes of Newcastle disease virus (NDV) incorporated with chicken interferon(provax-chIFN-gamma) cDNA as a molecular adjuvant and levamisole (LMS) as a chemical adjuvant was tested for its efficacy in protection against NDV lethal challenge. Compared with DNA vaccine alone, the DNA vaccine with provax-chIFN-gamma plus LMS induced significantly higher humoral and cell-mediated responses, as shown by higher levels of hemagglutination inhibition (HI) titers and T cell proliferation. In addition, the DNA vaccine with provax-chIFN-gamma plus LMS formulation increased the expression of IFN-gamma, interleukin (IL)-2, IL-4, IL-12, and IL-13, suggesting that the effectiveness of the IFN-gamma and LMS formulation is partly due to the enhancement of balanced cytokine production. Furthermore, the two adjuvants yielded 80% protection in chickens against challenge with a lethal dose of the virulent NDV strain. This study demonstrates that the synergistic effects of provax-chIFN-gamma plus LMS as the adjuvants in NDV DNA vaccination could be used to improve protective efficacy in chickens.
体液免疫和细胞介导的免疫反应对于保护动物免受初始急性病毒感染和建立慢性感染都很重要。DNA疫苗的佐剂可以影响体液免疫和细胞介导免疫之间的平衡。在本研究中，测试了一种编码新城疫病毒（NDV）血凝素-神经氨酸酶和融合基因的DNA疫苗，该疫苗与作为分子佐剂的鸡干扰素（provax-chIFN-γ）cDNA和作为化学佐剂的左旋咪唑（LMS）结合，以评估其对NDV致死性攻击的保护效果。与单独的DNA疫苗相比，含有provax-chIFN-γ加LMS的DNA疫苗诱导了显著更高的体液免疫和细胞介导反应，血凝抑制（HI）滴度和T细胞增殖水平更高表明了这一点。此外，含有provax-chIFN-γ加LMS配方的DNA疫苗增加了IFN-γ、白细胞介素（IL）-2、IL-4、IL-12和IL-13的表达，这表明IFN-γ和LMS配方的有效性部分归因于平衡细胞因子产生的增强。此外，这两种佐剂在鸡中对致死剂量的强毒NDV株攻击产生了80%的保护作用。本研究表明，provax-chIFN-γ加LMS作为NDV DNA疫苗佐剂的协同效应可用于提高鸡的保护效果。
