Nagy E, Krell P J, Dulac G C, Derbyshire J B
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
Avian Dis. 1991 Jul-Sep;35(3):585-90.
Vaccination of chickens with an oil-emulsion vaccine containing a recombinant baculovirus that expressed the hemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV)-induced hemagglutination-inhibition (HI) and virus-neutralizing antibodies against NDV. HI antibody titers obtained in response to vaccination with the live recombinant virus were higher than those obtained when the recombinant was inactivated with beta-propiolactone, and the titers were lower than those obtained in response to the same HN concentrations in live or beta-propiolactone-inactivated NDV strain B1. The serological response to the recombinant baculovirus was differentiated from the response to NDV by an enzyme-linked immunosorbent assay in which purified NDV nucleoprotein was used as antigen. Chickens vaccinated with the live recombinant or with inactivated NDV resisted an oculonasal challenge with the neurotropic velogenic Texas GB strain of NDV, which was lethal in unvaccinated controls. It was concluded that the HN protein of NDV expressed as a subunit by a recombinant baculovirus was protective against Newcastle disease.
用含有表达新城疫病毒（NDV）血凝素 - 神经氨酸酶（HN）的重组杆状病毒的油乳剂疫苗对鸡进行接种，可诱导产生针对NDV的血凝抑制（HI）和病毒中和抗体。接种活重组病毒后获得的HI抗体效价高于用β-丙内酯灭活重组病毒后获得的效价，且该效价低于在活的或β-丙内酯灭活的NDV B1株中相同HN浓度下获得的效价。通过以纯化的NDV核蛋白为抗原的酶联免疫吸附测定，区分了对重组杆状病毒的血清学反应和对NDV的反应。用活重组体或灭活NDV接种的鸡抵抗了NDV嗜神经速发型德州GB株的眼鼻接种攻击，该毒株在未接种的对照鸡中是致死性的。得出的结论是，由重组杆状病毒表达为亚单位的NDV的HN蛋白对新城疫具有保护作用。
