Delbari Ali, Bayat Mohammad, Bayat Maryam
Avicenna Hospital, Tehran, Iran.
Photomed Laser Surg. 2007 Jun;25(3):191-6. doi: 10.1089/pho.2007.2028.
This study sought to investigate whether low-level laser therapy (LLLT) with a helium-neon (He-Ne) laser would increase fibril diameter of transected medial collateral ligament (MCL) in rats.
It has been shown that LLLT can increase ultimate tensile strength MCL healing.
Thirty rats received surgical transect to their right MCL, and five were assigned as the control group. After surgery, the rats were divided into three groups: group 1 (n = 10) received LLLT with He-Ne laser and 0.01 J/cm(2) energy fluency per day, group 2 (n = 10) received LLLT with 1.2 J/cm(2) energy fluency (density) per day and group 3 (sham-exposed group; n = 10) received daily placebo laser with shut-down laser equipment, while control group received neither surgery nor LLLT. Transmission electron microscope (TEM) examination was performed on days 12 and 21 after surgery and dimension and density of ligament fibrils were measured. The data were analyzed by Student t-test and Mann-Whitney tests, respectively.
On day 12, the fibril dimension of group 2 and their density were higher than of groups 1 and 3.
LLLT with He-Ne laser on incised MCL in rats could not significantly increase fibril diameter and their density in comparison with sham-exposed group.
本研究旨在调查氦氖（He-Ne）激光低强度激光疗法（LLLT）是否会增加大鼠横断内侧副韧带（MCL）的纤维直径。
已有研究表明，LLLT可提高MCL愈合的极限拉伸强度。
30只大鼠右侧MCL接受手术横断，5只作为对照组。术后，大鼠被分为三组：第1组（n = 10）每天接受He-Ne激光LLLT治疗，能量通量为0.01 J/cm²；第2组（n = 10）每天接受能量通量（密度）为1.2 J/cm²的LLLT治疗；第3组（假照射组；n = 10）每天接受关闭激光设备的安慰剂激光照射，而对照组既不接受手术也不接受LLLT治疗。术后第１２天和第２１天进行透射电子显微镜（TEM）检查，并测量韧带纤维的尺寸和密度。数据分别采用Student t检验和Mann-Whitney检验进行分析。
术后第12天，第2组的纤维尺寸及其密度高于第1组和第3组。
与假照射组相比，He-Ne激光对大鼠切开的MCL进行LLLT治疗并不能显著增加纤维直径及其密度。
