Javadieh Farshad, Bayat Mohammad, Torkaman Giti
Medical Faculty, Shahid Beheshti University, MC, Tehran, Iran.
Photomed Laser Surg. 2010 Jun;28(3):423-8. doi: 10.1089/pho.2008.2434.
The aim of the present study was to examine the influence of low-level laser therapy (LLLT) on the healing of an osteochondral defect in rabbits.
Full-thickness defects of articular cartilage in the knee have a negligible capacity to heal spontaneously. Biostimulatory effects of LLLT on chondrocytes and articular cartilage were reported.
An osteochondral defect with a 5-mm diameter was surgically induced in the right femoral patellar groove of forty-two adult male rabbits. They were divided into control and experimental groups. The experimental group received He-Ne laser treatment (632.8 nm, 10 mW, circular laser beam, with a surface area of 0.0314 cm(2)) of 148.4 J/cm(2) three times a week. The control group received placebo LLLT with the equipment switched off. Each group was divided into four subgroups: A, B, C, and D. The subgroups were sacrificed at 2, 4, 8, and 16 wk after surgery, respectively. The femur was removed and the defect was examined biomechanically by the in-situ indentation method. The instantaneous stiffness, maximum force, equilibrium load, and energy absorption were calculated.
The results of the experimental group were higher than those of the control group. Only one significant difference was observed between the control and experimental groups: the second part of the energy absorption of the experimental group (56.11 +/- 11.5) was significantly higher than that of the control group (18.9 +/- 4) (p = 0.015) 4 wk after surgery.
LLLT with a He-Ne laser of an osteochondral defect in rabbits could not significantly accelerate the healing of the osteochondral defect from a biomechanical standpoint when compared with a control group.
本研究旨在探讨低强度激光疗法（LLLT）对兔骨软骨缺损愈合的影响。
膝关节全层关节软骨缺损自发愈合能力微乎其微。已有报道称LLLT对软骨细胞和关节软骨具有生物刺激作用。
在42只成年雄性兔的右股骨髌沟处手术制造一个直径5毫米的骨软骨缺损。将它们分为对照组和实验组。实验组每周接受3次氦氖激光治疗（632.8纳米，10毫瓦，圆形激光束，表面积0.0314平方厘米），能量密度为148.4焦/平方厘米。对照组在设备关闭的情况下接受安慰剂LLLT治疗。每组再分为四个亚组：A、B、C和D。分别在术后2、4、8和16周处死各亚组。取出股骨，采用原位压痕法对缺损进行生物力学检测。计算瞬时刚度、最大力、平衡载荷和能量吸收。
实验组的结果高于对照组。对照组和实验组之间仅观察到一个显著差异：术后4周时，实验组的能量吸收第二部分（56.11±11.5）显著高于对照组（18.9±4）（p = 0.015）。
与对照组相比，用氦氖激光进行LLLT对兔骨软骨缺损从生物力学角度而言并不能显著加速其愈合。
