随机扩增多态性DNA（RAPD）分析与ATB ID 32C系统在鉴定不同念珠菌临床分离株中的比较。

Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species.

作者信息

Baires-Varguez Laura, Cruz-García Alejandro, Villa-Tanaka Lourdes, Sánchez-García Sergio, Gaitán-Cepeda Luis Alberto, Sánchez-Vargas Luis Octavio, Quindós Guillermo, Hernández-Rodríguez César

机构信息

Facultad de Odontología, Universidad Nacional Autónoma de México (UNAM), 06400 Mexico City, Mexico.

出版信息

Rev Iberoam Micol. 2007 Jun;24(2):148-51. doi: 10.1016/s1130-1406(07)70031-1.

DOI:10.1016/s1130-1406(07)70031-1

PMID:17604435

Abstract

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.

摘要

本研究的目的是比较随机扩增多态性DNA（RAPD）分析与ATB ID32C试剂盒（法国生物梅里埃公司）对从临床标本中分离出的不同念珠菌属物种进行鉴定的实用性。用OPE - 18引物获得的用于鉴定临床分离株的RAPD - PCR图谱是一致的，不同的独立分析显示条带模式可重复。用OPE - 18引物进行RAPD分析是一种非常特异且灵敏的方法，可用于鉴定光滑念珠菌、季也蒙念珠菌、热带念珠菌、膜醭念珠菌、白色念珠菌、克柔念珠菌和葡萄牙念珠菌。