利用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）技术对医院获得性念珠菌血症分离出的念珠菌属进行鉴别。

Differentiation of Candida species obtained from nosocomial candidemia using RAPD-PCR technique.

作者信息

Valério Henrique Maia, Weikert-Oliveira Rita de Cássia Botelho, Resende Maria Aparecida de

机构信息

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

出版信息

Rev Soc Bras Med Trop. 2006 Mar-Apr;39(2):174-8. doi: 10.1590/s0037-86822006000200008. Epub 2006 May 5.

DOI:10.1590/s0037-86822006000200008

PMID:16699645

Abstract

Thirteen strains of the genus Candida were isolated from catheter, urine and surgical wounds from individual patients of the Santa Casa de Misericórdia, Belo Horizonte, MG, Brazil. Ten strains were characterized as Candida albicans, two as Candida glabrata, and one as Candida parapsilosis. Isolates were evaluated for molecular relatedness by random amplified polymorphic DNA technique using 15 primers. The analysis of the genomic DNA obtained revealed a low intraspecific polymorphism and did not allow for the differentiation between strains of the same species obtained from distinct clinical sources (catheter, urine and surgical wounds). The RAPD profiles generated were able to differentiate among the species of Candida albicans, Candida parapsilosis and Candida glabrata strains isolated in this study.

摘要

从巴西米纳斯吉拉斯州贝洛奥里藏特市圣卡塔琳娜慈善医院的个体患者的导管、尿液和手术伤口中分离出13株念珠菌属菌株。其中10株鉴定为白色念珠菌，2株为光滑念珠菌，1株为近平滑念珠菌。使用15种引物通过随机扩增多态性DNA技术对分离株进行分子相关性评估。对获得的基因组DNA的分析显示种内多态性较低，无法区分从不同临床来源（导管、尿液和手术伤口）获得的同一物种的菌株。本研究中产生的RAPD图谱能够区分白色念珠菌、近平滑念珠菌和光滑念珠菌菌株。