原发性胆汁性肝硬化中抗磷脂抗体的高频率。
High frequency of antiphospholipid antibodies in primary biliary cirrhosis.
作者信息
Mankaï Amani, Manoubi Wiem, Ghozzi Mariam, Melayah Sarra, Sakly Wahiba, Ghedira Ibtissem
机构信息
Research Unit (03/UR/07-02), Faculty of Pharmacy, Monastir University, Monastir, Tunisia; High School of Sciences and Techniques of Health, Tunis el Manar University, Tunis, Tunisia.
出版信息
J Clin Lab Anal. 2015 Jan;29(1):32-6. doi: 10.1002/jcla.21723. Epub 2014 Mar 28.
AIM
To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC).
PATIENTS AND METHODS
We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL IgG, IgA, and IgM) and beta 2 glycoprotein I (aβ2GPI IgG, IgA, and IgM).
RESULTS
The frequency of antiphospholipid antibodies (aCL and/or aβ2GPI) was significantly higher in PBC patients than in controls (70 vs. 5%, P < 10(-6)). The frequency of aCL antibodies (IgG, IgA or IgM) was significantly higher in PBC patients than in the control group (23.7 vs. 3.7%, P = 0.0005). The frequencies of aCL IgA and aCL IgM in PBC patients' sera were significantly higher than those in the control group (10 vs. 0%, P = 0.003 and 20 vs. 2.5%, P = 0.001, respectively). Two patients of eighty (2.5%) had aCL IgG, aCL IgA and aCL IgM. The frequency of aβ2GPI antibodies (IgG, IgA, or IgM) was significantly higher in PBC patients than in the control group (70 vs. 1.2%, P < 10(-6)). The frequencies of aβ2GPI IgG, aβ2GPI IgA, and aβ2GPI IgM in PBC patients' sera were significantly higher in patients than in the control group (12.5 vs. 0%, P = 0.003; 62.5 vs. 1.2%, P < 10(-6); and 21.2 vs. 0%, P < 10(-4), respectively).
CONCLUSION
Autoantibodies related to APLS (aCL and aβ2GPI) were present in the majority of patients with PBC, reflecting the ability of these antibodies to engage mediators of damage.
目的
回顾性评估突尼斯原发性胆汁性肝硬化（PBC）患者中抗磷脂综合征（APLS）自身抗体的出现频率。
患者与方法
我们分析了80例PBC患者的血清以及80例献血者的血清。采用酶联免疫吸附测定（ELISA）法测定抗心磷脂抗体（aCL IgG、IgA和IgM）及β2糖蛋白I抗体（aβ2GPI IgG、IgA和IgM）的出现频率。
结果
PBC患者中抗磷脂抗体（aCL和/或aβ2GPI）的出现频率显著高于对照组（70%对5%，P<10⁻⁶）。PBC患者中aCL抗体（IgG、IgA或IgM）的出现频率显著高于对照组（23.7%对3.7%，P = 0.0005）。PBC患者血清中aCL IgA和aCL IgM的出现频率显著高于对照组（分别为10%对0%，P = 0.003；20%对2.5%，P = 0.001）。80例患者中有2例（2.5%）同时出现aCL IgG、aCL IgA和aCL IgM。PBC患者中aβ2GPI抗体（IgG、IgA或IgM）的出现频率显著高于对照组（70%对1.2%，P<10⁻⁶）。PBC患者血清中aβ2GPI IgG、aβ2GPI IgA和aβ2GPI IgM的出现频率显著高于对照组（分别为12.5%对0%，P = 0.003；62.5%对1.2%，P<10⁻⁶；21.2%对0%，P<10⁻⁴）。
结论
大多数PBC患者体内存在与APLS相关的自身抗体（aCL和aβ2GPI），这反映出这些抗体具有介导损伤的能力。
Zotero 插件