Hayashi Y
Department of Physiology, Okayama University Medical School, Japan.
Brain Res. 1991 Sep 27;560(1-2):181-5. doi: 10.1016/0006-8993(91)91230-x.
In our previous study, an increase in concentration of putrescine was observed in the brain of amygdaloid kindled rats. To clarify the role of putrescine in kindling, the effects of intra-amygdaloid injections of alpha-difluoromethylornithine (DFMO), an inhibitor of polyamine synthesis, or putrescine on the development of electrical kindling were examined in rats. Kindling stimulations were applied to the left amygdala. Pretreatment with DFMO (50 nmol) for 10 days by injection into the ipsilateral amygdala decreased both the number of stimulations and total afterdischarge (AD) duration required to reach first fully kindled state. Kindling-induced elevation of the putrescine concentration was inhibited by pretreatment with this dose of DFMO. On the other hand, pretreatment with putrescine (200 nmol) for 10 days into the same site of the brain retarded the behavioral and electrographic development of kindling seizure during the period of drug administration, although these treatments did not produce any changes in the number of stimulations or total AD duration. Putrescine (200 nmol) demonstrated no anticonvulsive effects in previously kindled animals. These results suggest that putrescine has an inhibitory effect on the development of amygdaloid kindling in rats.
在我们之前的研究中，观察到杏仁核点燃大鼠的大脑中腐胺浓度增加。为了阐明腐胺在点燃过程中的作用，我们在大鼠中研究了向杏仁核内注射多胺合成抑制剂α-二氟甲基鸟氨酸（DFMO）或腐胺对电点燃发展的影响。点燃刺激施加于左侧杏仁核。通过向同侧杏仁核注射DFMO（50 nmol）预处理10天，可减少达到首次完全点燃状态所需的刺激次数和总后放电（AD）持续时间。用该剂量的DFMO预处理可抑制点燃诱导的腐胺浓度升高。另一方面，在大脑同一部位用腐胺（200 nmol）预处理10天，在给药期间会延缓点燃性癫痫发作的行为和脑电图发展，尽管这些处理对刺激次数或总AD持续时间没有产生任何变化。腐胺（200 nmol）在先前点燃的动物中未显示出抗惊厥作用。这些结果表明，腐胺对大鼠杏仁核点燃的发展具有抑制作用。
