Hayashi Y, Hattori Y, Hori Y
Department of Physiology, Okayama University Medical School, Japan.
J Neurochem. 1992 Feb;58(2):562-6. doi: 10.1111/j.1471-4159.1992.tb09756.x.
During the development of kindling by daily electrical stimulations applied to the left amygdala of rats, concentrations of the polyamines putrescine, spermidine, and spermine were measured in the left amygdala and the remainder of the cerebrum. A significant increase of putrescine concentration appeared first at the left amygdala in prekindled rats and then propagated to the remainder of the cerebrum with the development of kindling. This increase in putrescine concentration in the left amygdala was higher in prekindled rats than in fully kindled rats and lasted for at least 24 h after the final kindling stimulation. The concentrations of spermidine and spermine were slightly increased in a fully kindled state. To clarify the role of putrescine in kindling, the development of amygdaloid kindling was examined in rats after microinjections of alpha-difluoromethylornithine, a specific inhibitor of polyamine synthesis, and putrescine into the ipsilateral amygdala. Pretreatment with alpha-difluoromethylornithine (50 nmol) for 10 days accelerated both the development of behavioral kindling and the propagation of the afterdischarge from the left amygdala to the frontal cortex. In contrast, pretreatment with putrescine (200 nmol) for 10 days retarded the development of kindling. These results suggest that the increase in putrescine concentration in the kindled brain has an inhibitory effect on the development of kindling.
在通过每日对大鼠左侧杏仁核施加电刺激来诱导点燃的过程中，测定了左侧杏仁核和大脑其余部分中多胺腐胺、亚精胺和精胺的浓度。在预点燃大鼠中，腐胺浓度首先在左侧杏仁核显著升高，然后随着点燃的发展传播到大脑其余部分。预点燃大鼠左侧杏仁核中腐胺浓度的升高高于完全点燃的大鼠，并且在最后一次点燃刺激后至少持续24小时。在完全点燃状态下，亚精胺和精胺的浓度略有升高。为了阐明腐胺在点燃中的作用，在向同侧杏仁核微量注射多胺合成的特异性抑制剂α-二氟甲基鸟氨酸和腐胺后，检查了大鼠杏仁核点燃的发展情况。用α-二氟甲基鸟氨酸（50 nmol）预处理10天加速了行为点燃的发展以及放电从左侧杏仁核向额叶皮质的传播。相反，用腐胺（200 nmol）预处理10天延缓了点燃的发展。这些结果表明，点燃脑中腐胺浓度的增加对点燃的发展具有抑制作用。
