多形汉逊酵母过氧化物酶体突变体中过氧化物酶体结构的比较研究
A comparative study of peroxisomal structures in Hansenula polymorpha pex mutants.
作者信息
Koek Anne, Komori Masayuki, Veenhuis Marten, van der Klei Ida J
机构信息
Laboratory of Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
出版信息
FEMS Yeast Res. 2007 Oct;7(7):1126-33. doi: 10.1111/j.1567-1364.2007.00261.x. Epub 2007 Jun 30.
In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis (PEX genes) in various fungi. We have now performed a systematic study of the morphology of peroxisome remnants ('ghosts') in Hansenula polymorpha pex mutants (pex1-pex20) and the level of peroxins and matrix proteins in these strains. To this end, all available H. polymorpha pex strains were grown under identical cultivation conditions in glucose-limited chemostat cultures and analyzed in detail. The H. polymorpha pex mutants could be categorized into four distinct groups, namely pex mutants containing: (1) virtually normal peroxisomal structures (pex7, pex17, pex20); (2) small peroxisomal membrane structures with a distinct lumen (pex2, pex4, pex5, pex10, pex12, pex14); (3) multilayered membrane structures lacking apparent matrix protein content (pex1, pex6, pex8, pex13); and (4) no peroxisomal structures (pex3, pex19).
在最近的一项研究中，我们对多种真菌中参与过氧化物酶体生物发生的基因（PEX基因）的保守性进行了系统的基因组分析。我们现在对多形汉逊酵母pex突变体（pex1 - pex20）中过氧化物酶体残余物（“幽灵”）的形态以及这些菌株中过氧化物酶和基质蛋白的水平进行了系统研究。为此，所有可用的多形汉逊酵母pex菌株在葡萄糖限制的恒化器培养中在相同的培养条件下生长并进行了详细分析。多形汉逊酵母pex突变体可分为四个不同的组，即含有以下物质的pex突变体：（1）几乎正常的过氧化物酶体结构（pex7、pex17、pex20）；（2）具有明显内腔的小过氧化物酶体膜结构（pex2、pex4、pex5、pex10、pex12、pex14）；（3）缺乏明显基质蛋白含量的多层膜结构（pex1、pex6、pex8、pex13）；以及（4）没有过氧化物酶体结构（pex3、pex19）。
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