多形汉逊酵母过氧化物酶体缺陷型突变体中的过氧化物酶体残余物[已修正]

Peroxisomal remnants in peroxisome-deficient mutants of the yeast Hansenula polymorpha [corrected].

作者信息

Veenhuis M, Komori M, Salomons F, Hilbrands R E, Hut H, Baerends R J, Kiel J A, van der Klei I J

机构信息

Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.

出版信息

FEBS Lett. 1996 Mar 25;383(1-2):114-8. doi: 10.1016/0014-5793(96)00220-7.

DOI:10.1016/0014-5793(96)00220-7

PMID:8612776

Abstract

We have analyzed the presence of peroxisomal remnants ('ghosts') in three peroxisome-deficient (per) mutants of the yeast Hansenula polymorpha, namely delta per4, delta per5 and delta per10. Under peroxisome-inducing growth conditions peroxisomal membrane proteins (PMPs) were normally synthesized in cells of these mutants. In addition, these cells contained clusters of small membraneous vesicles, which were absent in cells grown under peroxisome-repressing growth conditions. These structures displayed typical peroxisomal properties in that they proliferated upon overproduction of Per8p, the H. polymorpha peroxisome proliferation factor. Moreover, in delta per4 and delta per5 these vesicles were susceptible to glucose-induced proteolytic degradation.

摘要

我们分析了多形汉逊酵母的三个过氧化物酶体缺陷（per）突变体，即Δper4、Δper5和Δper10中过氧化物酶体残余物（“幽灵”）的存在情况。在过氧化物酶体诱导生长条件下，这些突变体细胞中过氧化物酶体膜蛋白（PMPs）正常合成。此外，这些细胞含有小膜泡簇，而过氧化物酶体抑制生长条件下生长的细胞中不存在这些小膜泡簇。这些结构具有典型的过氧化物酶体特性，因为它们在多形汉逊酵母过氧化物酶体增殖因子Per8p过量产生时会增殖。此外，在Δper4和Δper5中，这些小泡易受葡萄糖诱导的蛋白水解降解作用。