夏枯草对肥大细胞介导的过敏反应及炎性细胞因子产生的影响。
Effects of Prunella vulgaris on mast cell-mediated allergic reaction and inflammatory cytokine production.
作者信息
Kim Sang-Yong, Kim Sang-Hyun, Shin Hye-Young, Lim Jong-Pil, Chae Byeong-Suk, Park Jeong-Suk, Hong Seong-Gyun, Kim Myung-Soo, Jo Dong-Gwang, Park Won-Hwan, Shin Tae-Yong
机构信息
Division of Specimen and Genetic Resources, Korea National Arboretum, Pocheon, Gyeonggi, South Korea.
出版信息
Exp Biol Med (Maywood). 2007 Jul;232(7):921-6.
In this study, we investigated the effect of aqueous extract of Prunella vulgaris (Labiatae; PVAE) on the mast cell-mediated allergy model. We found that PVAE (0.001-0.1 g/kg) dose dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. PVAE decreased the IgE-mediated local allergic reaction, passive cutaneous anaphylaxis. In addition, PVAE attenuated phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-stimulated TNF-alpha, IL-6, and IL-8 secretion in human mast cells. The inhibitory effect of PVAE on proinflammatory cytokines was nuclear factor-kappaB (NF-kappaB) dependent. PVAE suppressed PMA and A23187-induced NF-kappaB/DNA binding activity and NF-kappaB-dependent gene reporter assay. Our findings provide evidence that PVAE inhibits mast cell-derived immediate-type allergic reactions and involvement of proinflammatory cytokines and NF-kappaB in these effects.
在本研究中，我们调查了夏枯草水提取物（唇形科；PVAE）对肥大细胞介导的过敏模型的影响。我们发现，PVAE（0.001 - 0.1 g/kg）剂量依赖性地抑制化合物48/80诱导的小鼠全身过敏反应和血清组胺释放。PVAE减少了IgE介导的局部过敏反应，即被动皮肤过敏反应。此外，PVAE减弱了佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和钙离子载体A23187刺激的人肥大细胞中TNF-α、IL-6和IL-8的分泌。PVAE对促炎细胞因子的抑制作用依赖于核因子-κB（NF-κB）。PVAE抑制了PMA和A23187诱导的NF-κB/DNA结合活性以及NF-κB依赖性基因报告分析。我们的研究结果提供了证据，表明PVAE抑制肥大细胞衍生的速发型过敏反应以及促炎细胞因子和NF-κB参与这些效应。
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