Kim Sang-Hyun, Kwon Taeg Kyu, Shin Tae-Yong
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, South Korea.
Exp Biol Med (Maywood). 2008 Feb;233(2):192-9. doi: 10.3181/0708-RM-222.
In this study, we investigated the effect of the methanol extract of fruits of Vitis amurensis Rupr. (Vitaceae; MEVA) on the mast cell-mediated allergy model and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases, such as asthma and sinusitis. The discovery of drugs for the treatment of allergic disease is an important subject in human health. MEVA inhibited compound 48/80-induced systemic reactions and serum histamine release in a dose-dependent manner in mice. MEVA decreased immunoglobulin E (IgE)-mediated local allergic reactions, passive cutaneous anaphylaxis. MEVA dose-dependently reduced histamine release from mast cells activated by compound 48/80 or IgE. The inhibitory effect of MEVA on histamine release was mediated by the modulation of intracellular calcium. In addition, MEVA attenuated the phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-stimulated secretion of tumor necrosis factor-alpha, interleukin-6 (IL-6), and IL-8 in human mast cells. The inhibitory effect of MEVA on these proinflammatory cytokines was p38 mitogen-activated protein kinase and nuclear factor-kappaB (NF-kappaB) dependent. Our findings provide evidence that MEVA inhibits mast cell-derived, immediate-type allergic reactions and involvement of proinflammatory cytokines, p38 MAPK, and NF-kappaB in these effects.
在本研究中，我们研究了山葡萄（葡萄科；山葡萄甲醇提取物）果实的甲醇提取物对肥大细胞介导的过敏模型的影响，并探讨了其可能的作用机制。肥大细胞介导的过敏性疾病涉及多种疾病，如哮喘和鼻窦炎。发现治疗过敏性疾病的药物是人类健康领域的一个重要课题。山葡萄甲醇提取物在小鼠中以剂量依赖性方式抑制化合物48/80诱导的全身反应和血清组胺释放。山葡萄甲醇提取物减少了免疫球蛋白E（IgE）介导的局部过敏反应，即被动皮肤过敏反应。山葡萄甲醇提取物剂量依赖性地减少了由化合物48/80或IgE激活的肥大细胞中组胺的释放。山葡萄甲醇提取物对组胺释放的抑制作用是通过调节细胞内钙来介导的。此外，山葡萄甲醇提取物减弱了佛波醇12-肉豆蔻酸酯13-乙酸酯和钙离子载体A23187（PMACI）刺激的人肥大细胞中肿瘤坏死因子-α、白细胞介素-6（IL-6）和IL-8的分泌。山葡萄甲醇提取物对这些促炎细胞因子的抑制作用依赖于p38丝裂原活化蛋白激酶和核因子-κB（NF-κB）。我们的研究结果提供了证据，表明山葡萄甲醇提取物抑制肥大细胞衍生的速发型过敏反应以及促炎细胞因子、p38丝裂原活化蛋白激酶和NF-κB参与这些作用。
