van Erp S H, Kamenskaya E O, Khmelnitsky Y L
A. N. Bakh Institute of Biochemistry, Moscow, USSR.
Eur J Biochem. 1991 Dec 5;202(2):379-84. doi: 10.1111/j.1432-1033.1991.tb16385.x.
A simple theoretical model was suggested to describe quantitatively the effect of water content and nature of organic solvents on catalytic behavior of enzymes suspended in low-water media. The model was based on a generally accepted notion that the destruction of the protein hydration shell is one of the main reasons for protein denaturation by organic solvents. The validity of the model was confirmed by the example of catalytic behavior of immobilized laccase suspended in water/organic mixtures of different compositions. In addition, the results were used to demonstrate that the effect of organic solvents and/or water content on catalytic behavior of enzymes in low-water media can be adequately assessed only in terms of the full kinetic description based on properly determined Vm and Km values.
提出了一个简单的理论模型，用于定量描述水含量和有机溶剂性质对悬浮于低水介质中酶催化行为的影响。该模型基于一个普遍接受的观点，即蛋白质水合壳的破坏是有机溶剂导致蛋白质变性的主要原因之一。通过固定化漆酶悬浮于不同组成的水/有机混合物中的催化行为实例，证实了该模型的有效性。此外，这些结果还用于证明，只有基于适当确定的Vm和Km值进行完整的动力学描述，才能充分评估有机溶剂和/或水含量对低水介质中酶催化行为的影响。
