Liu Hao, Zhou Pandeng, Wu Xing, Sun Jianliang, Chen Shicheng
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
School of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, China.
Molecules. 2015 Nov 4;20(11):19907-13. doi: 10.3390/molecules201119672.
The biosynthetic utilization of laccase/mediator system is problematic because the use of organic cosolvent causes significant inhibition of laccase activity. This work explored how the organic cosolvent impacts on the laccase catalytic capacity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in aqueous solution. Effects of acetone on the kinetic constants of laccase were determined and the results showed Km and Vmax varied exponentially with increasing acetone content. Acetone as well as some other cosolvents could transform ABTS radicals into its reductive form. The content of acetone in media significantly affected the radical scavenging rates. Up to 95% of the oxidized ABTS was successfully recovered in 80% (v/v) acetone in 60 min. This allows ABTS recycles at least six times with 70%-75% of active radicals recovered after each cycle. This solvent-based recovery strategy may help improve the economic feasibility of laccase/ABTS system in biosynthesis.
漆酶/介体系统的生物合成应用存在问题，因为使用有机助溶剂会显著抑制漆酶活性。本研究探讨了有机助溶剂对漆酶在水溶液中催化2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)能力的影响。测定了丙酮对漆酶动力学常数的影响，结果表明Km和Vmax随丙酮含量的增加呈指数变化。丙酮以及其他一些助溶剂可将ABTS自由基转化为还原形式。介质中丙酮的含量显著影响自由基清除率。在60分钟内，80%(v/v)丙酮中95%的氧化ABTS被成功回收。这使得ABTS至少可以循环使用六次，每次循环后回收70%-75%的活性自由基。这种基于溶剂的回收策略可能有助于提高漆酶/ABTS系统在生物合成中的经济可行性。
