基于单壁碳纳米管与二茂铁加合物的DNA电化学传感器。

DNA electrochemical sensor based on an adduct of single-walled carbon nanotubes and ferrocene.

作者信息

Yang Xiaoying, Lu Yanhong, Ma Yanfeng, Liu Zunfeng, Du Feng, Chen Yongsheng

机构信息

School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin, China.

出版信息

Biotechnol Lett. 2007 Nov;29(11):1775-9. doi: 10.1007/s10529-007-9450-2. Epub 2007 Jul 5.

DOI:10.1007/s10529-007-9450-2

PMID:17611724

Abstract

A novel electrochemical sandwich-type gene sensing system was designed by using a DNA probe (DNA-probe1) immobilized on a gold electrode, the target DNA, and another DNA probe (DNA-probe2) conjugated on a single-walled carbon nanotubes/ferrocene (Fc-SWNT) adduct. In this sandwich-type gene-sensing electrode, the Fc-SWNT adduct could significantly amplify the electrochemical response of the reduction of H(2)O(2). The target DNA could be detected selectively and sensitively based on the much enhanced electrochemical catalytic property of the Fc-SWNT adduct toward H(2)O(2) reduction.

摘要

通过使用固定在金电极上的DNA探针（DNA-probe1）、目标DNA以及缀合在单壁碳纳米管/二茂铁（Fc-SWNT）加合物上的另一个DNA探针（DNA-probe2），设计了一种新型的电化学夹心型基因传感系统。在这种夹心型基因传感电极中，Fc-SWNT加合物能够显著放大H₂O₂还原的电化学响应。基于Fc-SWNT加合物对H₂O₂还原的大大增强的电化学催化性能，可以选择性且灵敏地检测目标DNA。