Gandini Gustavo, Pizzi Flavia, Stella Alessandra, Boettcher Paul J
Department VSA, Università degli Studi di Milano, Milan, Italy.
Genet Sel Evol. 2007 Jul-Aug;39(4):465-79. doi: 10.1186/1297-9686-39-4-465. Epub 2007 Jul 6.
The aim of this work was to compare costs, in the horse, cattle, sheep, swine, and rabbit species, for the creation of gene banks for reconstruction of an extinct breed, using different strategies: embryos-only, embryos in combination with semen, and semen-only. Three cost measures were used: time required for population reconstruction, cost for creation of the gene bank, number of years-keeping-female to reach reconstruction. Semen costs were estimated across four scenarios: the presence or absence of a commercial market for semen, purchase of semen donors, and semen extracted from the epididymus. The number of cells were doubled to take into account the creation of two storage sites. The strategy embryos-only required the shortest time to reach reconstruction. With the strategy embryos + semen, time increased with decreasing proportions of embryos. With semen-only, reconstruction time varied from 2 to 21 years. A high variation of costs was observed across species and strategies, from 360 Euros in the rabbit to 1,092,300 in the horse. In all species, the embryos-only strategy was about 10% more expensive than using 90% embryos + semen. Decreasing the percentage of embryos further diminished costs. The number of years-keeping-female ranged across strategies, from 2 in the rabbit, to a maximum of 12,878 in the horse.
这项工作的目的是比较马、牛、羊、猪和兔这几种物种，采用不同策略（仅胚胎、胚胎与精液结合、仅精液）建立用于重建灭绝品种的基因库的成本。使用了三种成本衡量指标：种群重建所需时间、基因库创建成本、达到重建所需的雌性饲养年限。精液成本在四种情况下进行估算：精液是否存在商业市场、购买精液供体以及从附睾中提取精液。细胞数量翻倍以考虑建立两个储存地点的情况。仅胚胎策略达到重建所需时间最短。采用胚胎 + 精液策略时，随着胚胎比例降低，时间增加。仅使用精液时，重建时间从2年到21年不等。观察到不同物种和策略的成本差异很大，从兔子的360欧元到马的1,092,300欧元。在所有物种中，仅胚胎策略比使用90%胚胎 + 精液的策略贵约10%。进一步降低胚胎百分比会降低成本。雌性饲养年限在不同策略中有所不同，从兔子的2年到马的最多12,878年。
