Zu Xiao-Yan, Xiong Guang-Quan, Geng Sheng-Rong, Liao Tao, Li Xin, Zhang Zhen-Ya
Institute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, P.R. China.
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Biomed Rep. 2014 May;2(3):388-391. doi: 10.3892/br.2014.259. Epub 2014 Mar 19.
This study was conducted to evaluate the sedative effects of L. stem and leaf extract (AHSLE) and determine its effect pathways through γ-aminobutyric acid (GABA)-gated channels on male Sprague-Dawley rats treated with pentobarbital. AHSLE was obtained from 98°C water (3 h, extracted twice). AHSLE and flumazenil (a GABA type A receptor antagonist) were administered to the rats orally, whereas pentobarbital sodium and muscimol (a GABA type A receptor agonist) were administered intraperitoneally (i.p.). The results demonstrated that AHSLE decreased sleep latency and increased sleep time in pentobarbital-treated rats (50 mg/kg, i.p.). The coadministration of AHSLE and muscimol (0.05 mg/kg) significantly increased sleep time and reduced sleep latency in pentobarbital-treated rats and these actions were significantly antagonized by flumazenil at a dose of 3.5 mg/kg. These results indicated that AHSLE improved the sleep behavior in pentobarbital-treated rats, possibly through GABA-gated channel-related mechanisms.
本研究旨在评估L. 茎叶提取物（AHSLE）的镇静作用，并确定其通过γ-氨基丁酸（GABA）门控通道对戊巴比妥处理的雄性Sprague-Dawley大鼠的作用途径。AHSLE通过98°C水（3小时，提取两次）获得。AHSLE和氟马西尼（一种GABA A型受体拮抗剂）经口给予大鼠，而戊巴比妥钠和蝇蕈醇（一种GABA A型受体激动剂）经腹腔注射（i.p.）。结果表明，AHSLE可缩短戊巴比妥处理大鼠（50 mg/kg，i.p.）的睡眠潜伏期并延长睡眠时间。AHSLE与蝇蕈醇（0.05 mg/kg）共同给药可显著延长戊巴比妥处理大鼠的睡眠时间并缩短睡眠潜伏期，而这些作用在3.5 mg/kg剂量的氟马西尼作用下被显著拮抗。这些结果表明，AHSLE可能通过与GABA门控通道相关的机制改善戊巴比妥处理大鼠的睡眠行为。
