Hojman Pernille, Eriksen Jens, Gehl Julie
Department of Oncology, Copenhagen University Hospital at Herlev, Herlev, Denmark.
Anim Biotechnol. 2007;18(3):183-8. doi: 10.1080/10495390601105055.
Gene transfer to skeletal muscle leads to long-term, stable expression of transferred genes. An exiting development is the use of inducible expression systems. Using the inducible Tet-On system, it has been customary to administer doxycycline in drinking water with added sucrose to ameliorate the bitter taste. During a study aiming at regulating electrotransferred genes through the Tet-On system, we observed excessive drinking behavior among mice. Removal of sugar from the drinking water led to normal drinking behavior and most importantly did not affect the level of gene expression. Based on this study, the practice of adding sucrose to drinking water in doxycycline induction studies should be abandoned.
基因转移至骨骼肌可导致转移基因的长期稳定表达。一项令人兴奋的进展是诱导表达系统的应用。使用诱导型Tet-On系统时，通常会在饮用水中添加蔗糖以改善苦味，从而便于小鼠饮用强力霉素。在一项旨在通过Tet-On系统调控电转移基因的研究中，我们观察到小鼠出现过度饮水行为。去除饮用水中的糖分后，小鼠饮水行为恢复正常，最重要的是，这并未影响基因表达水平。基于这项研究，在强力霉素诱导实验中向饮用水中添加蔗糖的做法应当摒弃。
