Tang Guanglin, Nierath Wiebke-Felicitas, Palme Rupert, Vollmar Brigitte, Zechner Dietmar
Rudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, Germany.
Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine, 1210 Vienna, Austria.
Animals (Basel). 2020 Dec 5;10(12):2306. doi: 10.3390/ani10122306.
Pain management during in vivo experiments is an animal welfare concern and is in many countries also legally required. In this study, we evaluated C57Bl/6J mice when 3 g/L metamizole or 1 g/L tramadol was provided via drinking water, before and during cerulein-induced chronic pancreatitis. Supplementation of drinking water with metamizole or tramadol did not significantly reduce the amount of consumed water. In order to evaluate the wellbeing of mice, a distress score, burrowing activity, nesting behavior, and body weight was assessed. Before induction of pancreatitis, neither tramadol nor metamizole influenced these readout parameters. Chronic pancreatitis caused a significantly increased distress score, decreased burrowing activity and a reduction in body weight. Mice drinking tramadol-supplemented water experienced less loss in body weight and consumed more water than mice drinking metamizole, at a few time-points during chronic pancreatitis. Pancreatic atrophy, a characteristic feature of chronic pancreatitis was not differentially influenced by either analgesic. In conclusion, both analgesics can be used during 33 days of chronic pancreatitis, but tramadol seems to be moderately advantageous when compared to metamizole.
体内实验期间的疼痛管理是一个动物福利问题，在许多国家也是法律要求的。在本研究中，我们在注射雨蛙肽诱导慢性胰腺炎之前和期间，评估了饮用含3 g/L安乃近或1 g/L曲马多的饮用水的C57Bl/6J小鼠。在饮用水中添加安乃近或曲马多并没有显著减少小鼠的饮水量。为了评估小鼠的健康状况，我们对小鼠的痛苦评分、打洞活动、筑巢行为和体重进行了评估。在诱导胰腺炎之前，曲马多和安乃近均未影响这些读数参数。慢性胰腺炎导致痛苦评分显著增加、打洞活动减少和体重下降。在慢性胰腺炎的几个时间点，饮用含曲马多的水的小鼠体重减轻较少，饮水量比饮用安乃近的小鼠更多。胰腺萎缩是慢性胰腺炎的一个特征性表现，两种镇痛药对其均无差异影响。总之，两种镇痛药均可在33天的慢性胰腺炎期间使用，但与安乃近相比，曲马多似乎具有一定优势。
