Steuerle H
J Chromatogr. 1975 Dec 24;115(2):447-53. doi: 10.1016/s0021-9673(01)98947-2.
Vitamin D3 and its isomers, prepared from 7-dehydrocholesterol by photochemical reaction, can be separated and quantitatively determined by high-pressure liquid chromatography on aluminium oxide using chloroform as the eluting agent. The determination of small amounts of vitamin D3 in an excess of vitamin A acetate can be achieved by the same method.
由7-脱氢胆固醇通过光化学反应制备的维生素D3及其异构体，可用氧化铝作固定相、氯仿作洗脱剂，通过高压液相色谱法进行分离和定量测定。在过量乙酸维生素A存在下，用同样方法可以测定少量的维生素D3。
