Lytton-Jean Abigail K R, Han Min Su, Mirkin Chad A
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
Anal Chem. 2007 Aug 1;79(15):6037-41. doi: 10.1021/ac070635h. Epub 2007 Jul 6.
We have designed a chip-based assay, using microarray technology, for determining the relative binding affinities of duplex and triplex DNA binders. This assay combines the high discrimination capabilities afforded by DNA-modified Au nanoparticles with the high-throughput capabilities of DNA microarrays. The detection and screening of duplex DNA binders are important because these molecules, in many cases, are potential anticancer agents as well as toxins. Triplex DNA binders are also promising drug candidates. These molecules, in conjunction with triplex-forming oligonucleotides, could potentially be used to achieve control of gene expression by interfering with transcription factors that bind to DNA. Therefore, the ability to screen for these molecules in a high-throughput fashion could dramatically improve the drug screening process. The assay reported here provides excellent discrimination between strong, intermediate, and weak duplex and triplex DNA binders in a high-throughput fashion.
我们设计了一种基于芯片的检测方法，利用微阵列技术来测定双链和三链DNA结合剂的相对结合亲和力。该检测方法将DNA修饰的金纳米颗粒所具有的高分辨能力与DNA微阵列的高通量能力结合起来。双链DNA结合剂的检测和筛选很重要，因为在许多情况下，这些分子既是潜在的抗癌剂也是毒素。三链DNA结合剂也是很有前景的候选药物。这些分子与形成三链的寡核苷酸一起，有可能通过干扰与DNA结合的转录因子来实现对基因表达的控制。因此，以高通量方式筛选这些分子的能力可以显著改善药物筛选过程。本文报道的检测方法能够以高通量方式出色地区分强、中、弱双链和三链DNA结合剂。
