Rcs和PhoPQ在调控肠炎沙门氏菌毒力方面存在调控重叠。
Rcs and PhoPQ regulatory overlap in the control of Salmonella enterica virulence.
作者信息
García-Calderón Clara B, Casadesús Josep, Ramos-Morales Francisco
机构信息
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, 6, 41012 Sevilla, Spain.
出版信息
J Bacteriol. 2007 Sep;189(18):6635-44. doi: 10.1128/JB.00640-07. Epub 2007 Jul 6.
Abstract
Genetic screens based on the use of MudJ-generated lac fusions permitted the identification of novel genes regulated by the Rcs signal transduction system in Salmonella enterica serovar Typhimurium. Besides genes that are also found in the Escherichia coli genome, our screens identified Salmonella-specific genes regulated by RcsB, including bapA, siiE, srfA, and srfB. Here we show that the srfABC operon is negatively regulated by RcsB and by PhoP. In vivo studies using mutants with constitutive activation of the Rcs and/or PhoPQ system suggested that there is an overlap between these regulatory systems in the control of Salmonella virulence.
摘要
基于使用 MudJ 产生的 lac 融合体的遗传筛选，使得在鼠伤寒沙门氏菌中能够鉴定出受 Rcs 信号转导系统调控的新基因。除了在大肠杆菌基因组中也存在的基因外，我们的筛选还鉴定出了受 RcsB 调控的沙门氏菌特异性基因，包括 bapA、siiE、srfA 和 srfB。在这里我们表明，srfABC 操纵子受到 RcsB 和 PhoP 的负调控。使用 Rcs 和/或 PhoPQ 系统组成型激活的突变体进行的体内研究表明，在沙门氏菌毒力控制方面，这些调控系统之间存在重叠。
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