比色微量稀释板杂交技术在22种分枝杆菌菌种快速基因鉴定中的应用
Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.
作者信息
Kusunoki S, Ezaki T, Tamesada M, Hatanaka Y, Asano K, Hashimoto Y, Yabuuchi E
机构信息
Research and Development Center, Kobayashi Pharmaceutical Co., Osaka, Japan.
出版信息
J Clin Microbiol. 1991 Aug;29(8):1596-603. doi: 10.1128/jcm.29.8.1596-1603.1991.
Abstract
Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated streptavidin and the substrate, tetramethylbenzidine. This method could differentiate among 20 of the 22 Mycobacterium species tested. The type strains of Mycobacterium tuberculosis and M. bovis were genetically highly related and could not be differentiated by this method. Of 194 biochemically identified human clinical strains, 178 (90%) were genetically identified within 3 h of the small-scale DNA extraction.
摘要
采用定量微量稀释板杂交法鉴定22种分枝杆菌。临床菌株的DNA被快速提取并用光反应性生物素标记。将标记的DNA分布到已固定参考DNA的微量稀释板孔中。杂交2小时后，用过氧化物酶偶联的链霉亲和素和底物四甲基联苯胺对杂交的DNA进行定量检测。该方法可以区分所测试的22种分枝杆菌中的20种。结核分枝杆菌和牛分枝杆菌的标准菌株在基因上高度相关，无法用该方法区分。在194株经生化鉴定的人类临床菌株中，178株（90%）在小规模DNA提取后3小时内得到基因鉴定。
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本文引用的文献
1
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J Gen Microbiol. 1982 Apr;128(4):817-22. doi: 10.1099/00221287-128-4-817.
2
Mycolic acid patterns of representative strains of Mycobacterium fortuitum, 'Myobacterium peregrinum' and Mycobacterium smegmatis.偶然分枝杆菌、“龟分枝杆菌”和耻垢分枝杆菌代表性菌株的分枝菌酸模式。
J Gen Microbiol. 1984 Feb;130(2):363-7. doi: 10.1099/00221287-130-2-363.
3
A co-operative numerical analysis of rapidly growing mycobacteria.快速生长分枝杆菌的合作数值分析。
J Gen Microbiol. 1972 Nov;73(1):55-70. doi: 10.1099/00221287-73-1-55.
4
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J Clin Microbiol. 1988 Sep;26(9):1708-13. doi: 10.1128/jcm.26.9.1708-1713.1988.
5
Repetitive DNA sequences as probes for Mycobacterium tuberculosis.作为结核分枝杆菌探针的重复DNA序列
J Clin Microbiol. 1988 Nov;26(11):2240-5. doi: 10.1128/jcm.26.11.2240-2245.1988.
6
Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex.使用Gen-Probe鸟分枝杆菌复合群和结核分枝杆菌复合群快速诊断系统从培养物中鉴定分枝杆菌。
J Clin Microbiol. 1988 Oct;26(10):2120-3. doi: 10.1128/jcm.26.10.2120-2123.1988.
7
Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes.使用DNA探针直接从BACTEC培养基中的扩增原代培养物中鉴定结核分枝杆菌、鸟分枝杆菌和胞内分枝杆菌。
J Clin Microbiol. 1989 Jul;27(7):1543-7. doi: 10.1128/jcm.27.7.1543-1547.1989.
8
Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov.依赖分枝杆菌素的分枝杆菌的数值分类学、鸟分枝杆菌的修订描述以及鸟分枝杆菌亚种鸟分枝杆菌亚种新组合、副结核分枝杆菌亚种新组合和林栖分枝杆菌亚种新组合的描述
Int J Syst Bacteriol. 1990 Jul;40(3):254-60. doi: 10.1099/00207713-40-3-254.
9
Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.使用高度特异性DNA探针和聚合酶链反应检测副结核分枝杆菌引起的副结核病。
J Clin Microbiol. 1990 May;28(5):933-7. doi: 10.1128/jcm.28.5.933-937.1990.
10
Evaluation of the microplate hybridization method for rapid identification of Legionella species.
Eur J Clin Microbiol Infect Dis. 1990 Mar;9(3):213-7. doi: 10.1007/BF01963841.
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