比色微量稀释板杂交技术在22种分枝杆菌菌种快速基因鉴定中的应用
Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.
作者信息
Kusunoki S, Ezaki T, Tamesada M, Hatanaka Y, Asano K, Hashimoto Y, Yabuuchi E
机构信息
Research and Development Center, Kobayashi Pharmaceutical Co., Osaka, Japan.
出版信息
J Clin Microbiol. 1991 Aug;29(8):1596-603. doi: 10.1128/jcm.29.8.1596-1603.1991.
Abstract
Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated streptavidin and the substrate, tetramethylbenzidine. This method could differentiate among 20 of the 22 Mycobacterium species tested. The type strains of Mycobacterium tuberculosis and M. bovis were genetically highly related and could not be differentiated by this method. Of 194 biochemically identified human clinical strains, 178 (90%) were genetically identified within 3 h of the small-scale DNA extraction.
摘要
采用定量微量稀释板杂交法鉴定22种分枝杆菌。临床菌株的DNA被快速提取并用光反应性生物素标记。将标记的DNA分布到已固定参考DNA的微量稀释板孔中。杂交2小时后，用过氧化物酶偶联的链霉亲和素和底物四甲基联苯胺对杂交的DNA进行定量检测。该方法可以区分所测试的22种分枝杆菌中的20种。结核分枝杆菌和牛分枝杆菌的标准菌株在基因上高度相关，无法用该方法区分。在194株经生化鉴定的人类临床菌株中，178株（90%）在小规模DNA提取后3小时内得到基因鉴定。
相似文献
1
Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.
J Clin Microbiol. 1991 Aug;29(8):1596-603. doi: 10.1128/jcm.29.8.1596-1603.1991.
2
Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells.
J Clin Microbiol. 1988 Sep;26(9):1708-13. doi: 10.1128/jcm.26.9.1708-1713.1988.
3
[Genetic identification of 25 Legionella species by modified microdilution plate hybridization, and its evaluation with isolated strains].
Kansenshogaku Zasshi. 1992 Jan;66(1):37-44. doi: 10.11150/kansenshogakuzasshi1970.66.37.
4
[Identification of Capnocytophaga species by microplate hybridization method, and its restriction endonuclease digestion patterns].
Rinsho Byori. 1995 Oct;43(10):1066-70.
5
Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria.
J Clin Microbiol. 1986 Nov;24(5):835-9. doi: 10.1128/jcm.24.5.835-839.1986.
6
Rolling circle amplification based colorimetric determination of Staphylococcus aureus.
Mikrochim Acta. 2020 Jan 11;187(2):119. doi: 10.1007/s00604-019-4082-5.
7
Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov.
Int J Syst Bacteriol. 1992 Apr;42(2):240-5. doi: 10.1099/00207713-42-2-240.
8
Rapid identification of Mycobacteria to the species level using INNO-LiPA Mycobacteria, a reverse hybridization assay.
J Clin Microbiol. 2001 Dec;39(12):4477-82. doi: 10.1128/JCM.39.12.4477-4482.2001.
9
PCR-enzyme-linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria.
J Clin Microbiol. 1997 Sep;35(9):2375-80. doi: 10.1128/jcm.35.9.2375-2380.1997.
10
[Rapid genetic identification system of mycobacteria].
Kekkaku. 1992 Dec;67(12):803-8.
引用本文的文献
1
Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification.
Front Public Health. 2024 Jun 19;12:1410672. doi: 10.3389/fpubh.2024.1410672. eCollection 2024.
2
Nontuberculous mycobacterial abscess of lacrimal sac and eyelid debridement: Case report.
Clin Case Rep. 2023 Dec 21;11(12):e8364. doi: 10.1002/ccr3.8364. eCollection 2023 Dec.
3
Synergistic Effects of Omadacycline with Other Antimicrobial Agents against Mycobacterium abscessus.
Antimicrob Agents Chemother. 2023 Jun 15;67(6):e0157922. doi: 10.1128/aac.01579-22. Epub 2023 May 8.
4
Diagnostic Utility of a Mycobacterium Multiplex PCR Detection Panel for Tuberculosis and Nontuberculous Mycobacterial Infections.
Microbiol Spectr. 2023 Jun 15;11(3):e0516222. doi: 10.1128/spectrum.05162-22. Epub 2023 Apr 24.
5
Mixed infection of three nontuberculous mycobacteria species identified by metagenomic next-generation sequencing in a patient with peritoneal dialysis-associated peritonitis: a rare case report and literature review.
BMC Nephrol. 2023 Apr 13;24(1):95. doi: 10.1186/s12882-023-03156-8.
6
Quantitative evaluation of Mycobacterium abscessus clinical isolate virulence using a silkworm infection model.
PLoS One. 2022 Dec 20;17(12):e0278773. doi: 10.1371/journal.pone.0278773. eCollection 2022.
7
Impact of lung cancer surgery on comorbid complex lung disease-A case series.
Respir Med Case Rep. 2022 May 4;37:101664. doi: 10.1016/j.rmcr.2022.101664. eCollection 2022.
8
Bursitis, Bacteremia, and Disseminated Infection of Mycobacteroides (Mycobacterium) abscessus subsp. massiliense.
Intern Med. 2021 Sep 15;60(18):3041-3045. doi: 10.2169/internalmedicine.6189-20. Epub 2021 Mar 29.
9
Peritoneal dialysis-associated peritonitis caused by Mycobacteroides massiliense: the first case and review of the literature.
BMC Nephrol. 2021 Mar 12;22(1):90. doi: 10.1186/s12882-021-02297-y.
10
Clinical risk factors related to treatment failure in Mycobacterium abscessus lung disease.
Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):247-254. doi: 10.1007/s10096-020-04026-z. Epub 2020 Sep 1.
本文引用的文献
1
The mycolic acids of Mycobacterium chelonei.
J Gen Microbiol. 1982 Apr;128(4):817-22. doi: 10.1099/00221287-128-4-817.
2
Mycolic acid patterns of representative strains of Mycobacterium fortuitum, 'Myobacterium peregrinum' and Mycobacterium smegmatis.
J Gen Microbiol. 1984 Feb;130(2):363-7. doi: 10.1099/00221287-130-2-363.
3
A co-operative numerical analysis of rapidly growing mycobacteria.
J Gen Microbiol. 1972 Nov;73(1):55-70. doi: 10.1099/00221287-73-1-55.
4
Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells.
J Clin Microbiol. 1988 Sep;26(9):1708-13. doi: 10.1128/jcm.26.9.1708-1713.1988.
5
Repetitive DNA sequences as probes for Mycobacterium tuberculosis.
J Clin Microbiol. 1988 Nov;26(11):2240-5. doi: 10.1128/jcm.26.11.2240-2245.1988.
6
Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex.
J Clin Microbiol. 1988 Oct;26(10):2120-3. doi: 10.1128/jcm.26.10.2120-2123.1988.
7
Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes.
J Clin Microbiol. 1989 Jul;27(7):1543-7. doi: 10.1128/jcm.27.7.1543-1547.1989.
8
Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov.
Int J Syst Bacteriol. 1990 Jul;40(3):254-60. doi: 10.1099/00207713-40-3-254.
9
Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.
J Clin Microbiol. 1990 May;28(5):933-7. doi: 10.1128/jcm.28.5.933-937.1990.
10
Evaluation of the microplate hybridization method for rapid identification of Legionella species.
Eur J Clin Microbiol Infect Dis. 1990 Mar;9(3):213-7. doi: 10.1007/BF01963841.
Zotero 插件