Minnikin D E, Minnikin S M, Goodfellow M, Stanford J L
J Gen Microbiol. 1982 Apr;128(4):817-22. doi: 10.1099/00221287-128-4-817.
Two-dimensional thin-layer chromatography of whole-organism acid methanolysates of Mycobacterium chelonei gives a characteristic pattern of two non-polar mycolic acid methyl esters which allows the organism to be distinguished from all other mycobacteria including Mycobacterium fortuitum. The mycolic acids from Mycobacterium chelonei were composed of approximately equal amounts of a diunsaturated alpha-mycolate and a lower molecular weight alpha'-mycolate, though minor amounts of a different alpha-mycolate were also detected. These mycolic acids are the first examples of natural mixtures from mycobacteria lacking major amounts of acids having oxygen functions in addition to the 3-hydroxy acid unit.
龟分枝杆菌全菌体酸性甲醇裂解产物的二维薄层色谱给出了两种非极性分枝菌酸甲酯的特征图谱，这使得该菌能够与包括偶然分枝杆菌在内的所有其他分枝杆菌区分开来。龟分枝杆菌的分枝菌酸由大约等量的二不饱和α-分枝菌酸酯和较低分子量的α'-分枝菌酸酯组成，不过也检测到少量不同的α-分枝菌酸酯。这些分枝菌酸是分枝杆菌天然混合物的首个例子，除了3-羟基酸单元外，缺乏大量具有氧功能的酸。
