Drouet E B, Denoyel G A, Boude M, Wallano E, Andujar M, de Montclos H P
Division of Infectious Diseases, Institut Pasteur de Lyon, France.
J Clin Microbiol. 1991 Aug;29(8):1620-4. doi: 10.1128/jcm.29.8.1620-1624.1991.
Immunoblotting experiments on hyperimmune rabbit serum and sera from patients with Helicobacter pylori gastritis showed a consistent antibody response to a 19-kDa outer membrane protein antigen. A monoclonal antibody, designated HP 40, which reacted by Western immunoblotting with this protein was produced. It was shared by all H. pylori strains tested (D 273, NCTC 11637, and 24 wild strains) but not by the thermophilic Campylobacter species, Campylobacter fetus, Helicobacter mustellae, or Helicobacter fennelliae. Immunogold staining suggested that the 19-kDa antigen was exposed on the outer surface of the bacteria. Its functional role and effectiveness as a serological diagnostic tool are under study.
对超免疫兔血清和幽门螺杆菌胃炎患者血清进行的免疫印迹实验表明，对一种19 kDa的外膜蛋白抗原有一致的抗体反应。制备了一种单克隆抗体，命名为HP 40，它通过Western免疫印迹与该蛋白发生反应。所有测试的幽门螺杆菌菌株（D 273、NCTC 11637和24株野生菌株）都有这种抗体，但嗜热弯曲菌、胎儿弯曲菌、鼬形螺杆菌或芬内螺杆菌没有。免疫金染色表明，19 kDa抗原暴露于细菌外表面。其功能作用以及作为血清学诊断工具的有效性正在研究中。
