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利用免疫球蛋白M单克隆抗体对幽门螺杆菌一种胞外多糖进行部分特性分析

Partial characterization of an external polysaccharide of Helicobacter pylori by using an immunoglobulin M monoclonal antibody.

作者信息

Drouet E B, De Montclos H P, Andujar M, Boude M, Grimaud J A, Denoyel G A

机构信息

Infectiology Unit, Institut Pasteur, Lyon, France.

出版信息

Infect Immun. 1993 Jun;61(6):2732-6. doi: 10.1128/iai.61.6.2732-2736.1993.

DOI:10.1128/iai.61.6.2732-2736.1993
PMID:7684730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC280910/
Abstract

A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant was located outside the bacterial cell, possibly in the outermost areas. This external reactivity was observed by immunofluorescence and immunoperoxidase assays and was confirmed by immunogold study at the ultrastructural level. The reactive epitope was formol and picric acid resistant and allowed the detection of the bacterium on fixed tissue biopsy specimens. The reactive component was extracted with phenol-water. Immunoblotting with such an antigen exhibited a clearly positive reactivity at a molecular mass between 50 and 120 kDa. This reactivity was suppressed by periodate oxidation, suggesting a carbohydrate epitope. The diagnostic value and significance of this polysaccharide in microbe-host interactions remain to be determined.

摘要

一种单克隆免疫球蛋白M抗体HP15/36，由小鼠骨髓瘤细胞系Sp2/O与用幽门螺杆菌D273（法国菌株）免疫的小鼠脾细胞融合制备的杂交瘤细胞系产生。对完整细菌和超薄切片的免疫电子显微镜检查表明，该决定簇位于细菌细胞外，可能在最外层区域。通过免疫荧光和免疫过氧化物酶测定观察到这种外部反应性，并通过超微结构水平的免疫金研究得到证实。反应性表位对甲醛和苦味酸具有抗性，并允许在固定组织活检标本上检测该细菌。反应成分用酚-水提取。用这种抗原进行免疫印迹在50至120 kDa的分子量处表现出明显的阳性反应性。这种反应性被高碘酸盐氧化所抑制，表明存在碳水化合物表位。这种多糖在微生物-宿主相互作用中的诊断价值和意义仍有待确定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/93995a454394/iai00018-0477-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/3c0a62657e88/iai00018-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/173c348c2116/iai00018-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/c307143676d0/iai00018-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/2a339dc0be50/iai00018-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/93995a454394/iai00018-0477-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/3c0a62657e88/iai00018-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/173c348c2116/iai00018-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/c307143676d0/iai00018-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/2a339dc0be50/iai00018-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb08/280910/93995a454394/iai00018-0477-b.jpg

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