Isenberg H D, D'Amato R F, Heifets L, Murray P R, Scardamaglia M, Jacobs M C, Alperstein P, Niles A
Division of Microbiology, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, New York 11042-1433.
J Clin Microbiol. 1991 Aug;29(8):1719-22. doi: 10.1128/jcm.29.8.1719-1722.1991.
A study to delineate the feasibility of a biphasic-culture approach for detection and isolation of mycobacteria from clinical specimens except blood was conducted in four medical centers. The biphasic system (Septi-Chek AFB, Roche Diagnostic Systems, Nutley, N.J.) was compared with conventional mycobacterial isolation media and the BACTEC system. Septi-Chek AFB showed the highest degree of mycobacterial recovery. In addition, Septi-Chek AFB consistently shortened the time required for recovery of mycobacteria from clinical specimens and supported the growth of small inoculum numbers of stock cultures of 14 mycobacterial species. The study indicates the feasibility and potential advantages of the biphasic approach for detection and isolation of mycobacteria.
一项关于双相培养法用于从除血液外的临床标本中检测和分离分枝杆菌的可行性研究在四个医疗中心开展。将双相系统(Septi-Chek AFB,罗氏诊断系统公司,新泽西州纳特利)与传统分枝杆菌分离培养基及BACTEC系统进行了比较。Septi-Chek AFB显示出最高的分枝杆菌回收率。此外,Septi-Chek AFB持续缩短了从临床标本中回收分枝杆菌所需的时间,并支持14种分枝杆菌菌株少量接种物的生长。该研究表明双相培养法用于检测和分离分枝杆菌具有可行性及潜在优势。