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罗氏分枝杆菌快速培养系统用于分枝杆菌分离的评估

Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria.

作者信息

D'Amato R F, Isenberg H D, Hochstein L, Mastellone A J, Alperstein P

机构信息

Department of Pathology, Catholic Medical Center of Brooklyn & Queens, Inc., Jamaica, New York 11432-3723.

出版信息

J Clin Microbiol. 1991 Dec;29(12):2906-8. doi: 10.1128/jcm.29.12.2906-2908.1991.

Abstract

The Septi-Chek AFB system for the recovery of mycobacteria from clinical specimens was compared with a conventional approach using Lowenstein-Jensen and Middlebrook 7H11 agars. A total of 1,532 clinical specimens were analyzed; 132 yielded mycobacteria. Mycobacterium tuberculosis and Mycobacterium avium complexes were the predominant isolates. With the conventional combination of Lowenstein-Jensen and 7H11 agars, 75.8% of the isolates were recovered; the Septi-Chek AFB allowed recovery of 100% of the isolates. Septi-Chek AFB required less time for the detection of mycobacteria than did the conventional media.

摘要

将用于从临床标本中分离分枝杆菌的Septi-Chek AFB系统与使用罗-琴培养基和Middlebrook 7H11琼脂的传统方法进行了比较。共分析了1532份临床标本;其中132份培养出了分枝杆菌。结核分枝杆菌和鸟分枝杆菌复合体是主要的分离菌株。使用罗-琴培养基和7H11琼脂的传统组合,75.8%的分离菌株被成功分离;而Septi-Chek AFB系统能分离出100%的菌株。与传统培养基相比,Septi-Chek AFB系统检测分枝杆菌所需的时间更短。

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