Hattori Mitsuru, Wazawa Tetsuichi, Orioka Mariko, Hiruta Yuki, Nagai Takeharu
Department of Biomolecular Science and Engineering, SANKEN, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
Sci Adv. 2025 Jan 24;11(4):eadp4750. doi: 10.1126/sciadv.adp4750. Epub 2025 Jan 22.
Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena.
生物发光是一种无需光激发的光学标记，它使研究人员能够同时观察多个目标，每个目标呈现出不同的颜色。值得注意的是，生物发光蛋白的颜色必须有足够的差异才能实现同时检测。在此，我们旨在引入一种方法，该方法可用于通过调节双受体生物发光共振能量转移来扩大颜色变化范围。使用这种方法，我们可以通过使用彩色互补金属氧化物半导体相机进行单次采集，以多达20种颜色可视化多个目标。总体而言，这种方法能够简单且同时地观察多个生物目标和现象。
